TY - JOUR
T1 - The herpes simplex virus type 1 ribonucleotide reductase is required for acute retinal disease
AU - Brandt, C. R.
AU - Imesch, P.
AU - Spencer, B.
AU - Eliassi-Rad, B.
AU - Syed, N. A.
AU - Untawale, S.
AU - Robinson, N. L.
AU - Albert, D. M.
PY - 1997
Y1 - 1997
N2 - We have used a herpes simplex virus type 1 (HSV-1) ribonucleotide reductase (RR) null mutant (ICP6Δ) to determine if the HSV-1 RR is required for acute retinal disease. Injection of the ICP6Δ mutant into the vitreous induced mild transient signs of infection (vitreal infiltrate, retinal inflammation, and changes in retinal cytology). In contrast, the parental KOS and a revertant virus (ICP6Δ + 3.1) in which the RR gene had been restored, caused severe retinitis. Injection of media alone also induced mild transient signs of disease. Two months after infection, ICP6Δ injected eyes could not be distinguished from normal eyes. Repeated injection of ICP6Δ (3 times, 2 weeks apart) resulted in vitreal infiltrate near the site of injection but the retina did not appear damaged. The mutant, ICP6Δ, grew to peak titers 1 x 103 to 1 x 105-fold lower and cleared faster than KOS or ICP6Δ + 3.1 in the injected eyes suggesting that the reduced virulence was due to reduced ability of the virus to grow. These results show that the viral RR is required for acute retinal disease.
AB - We have used a herpes simplex virus type 1 (HSV-1) ribonucleotide reductase (RR) null mutant (ICP6Δ) to determine if the HSV-1 RR is required for acute retinal disease. Injection of the ICP6Δ mutant into the vitreous induced mild transient signs of infection (vitreal infiltrate, retinal inflammation, and changes in retinal cytology). In contrast, the parental KOS and a revertant virus (ICP6Δ + 3.1) in which the RR gene had been restored, caused severe retinitis. Injection of media alone also induced mild transient signs of disease. Two months after infection, ICP6Δ injected eyes could not be distinguished from normal eyes. Repeated injection of ICP6Δ (3 times, 2 weeks apart) resulted in vitreal infiltrate near the site of injection but the retina did not appear damaged. The mutant, ICP6Δ, grew to peak titers 1 x 103 to 1 x 105-fold lower and cleared faster than KOS or ICP6Δ + 3.1 in the injected eyes suggesting that the reduced virulence was due to reduced ability of the virus to grow. These results show that the viral RR is required for acute retinal disease.
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U2 - 10.1007/s007050050126
DO - 10.1007/s007050050126
M3 - Article
C2 - 9191855
AN - SCOPUS:0031021047
VL - 142
SP - 883
EP - 896
JO - Archives of Virology
JF - Archives of Virology
SN - 0304-8608
IS - 5
ER -