The hemopexin-like C-terminal domain of membrane type 1 matrix metalloproteinase regulates proteolysis of a multifunctional protein, gC1qR

Dmitri Rozanov, Berhane Ghebrehiwet, Tatiana I. Postnova, Andreas Eichinger, Elena I. Deryugina, Alex Y. Strongin

Research output: Contribution to journalArticle

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Abstract

Matrix metalloproteinases (MMPs) including membrane type 1 MMP (MT1-MMP) can degrade extracellular matrix and cell surface receptor molecules and have an essential function in malignancy. Recently, we established a functional link between MT1-MMP and the receptor of complement component 1q (gC1qR). The gC1qR is known as a compartment-specific regulator of diverse cellular and viral proteins. Once released by proliferating cells, soluble gC1qR may inhibit complement component 1q hemolytic activity and play important roles in vivo in assisting tumor cells to evade destruction by complement. Here, we report that gC1qR is susceptible to MT1-MMP proteolysis in vitro and in cell cultures. The major MT1-MMP cleavage site (Gly79 ↓ G1n80) is localized within the structurally disordered loop connecting the β3and the β4 strands of gC1qR. The recombinant MT1-MMP construct that included the catalytic domain but lacked the hemopexin-like domain lost the proteolytic capacity; however, it retained the ability to bind gC1qR. Inhibition of MT1-MMP activity by a hydroxamate inhibitor converted the protease into a cell surface receptor of gC1qR and promoted co-precipitation MT1-MMP with the soluble gC1qR protein. It is tempting to hypothesize that these novel mechanisms may play important roles in vivo and have to be taken into account in designing hydroxamate-based cancer therapy.

Original languageEnglish (US)
Pages (from-to)9318-9325
Number of pages8
JournalJournal of Biological Chemistry
Volume277
Issue number11
DOIs
StatePublished - Mar 15 2002
Externally publishedYes

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Hemopexin
Proteolysis
Matrix Metalloproteinase 14
Matrix Metalloproteinases
Membranes
Complement C1q
Proteins
Cell Surface Receptors
Neoplasms
Viral Proteins
Coprecipitation
Protease Inhibitors
Cell culture
Extracellular Matrix
Tumors
Catalytic Domain
Cell Culture Techniques
Cells
Molecules

ASJC Scopus subject areas

  • Biochemistry

Cite this

The hemopexin-like C-terminal domain of membrane type 1 matrix metalloproteinase regulates proteolysis of a multifunctional protein, gC1qR. / Rozanov, Dmitri; Ghebrehiwet, Berhane; Postnova, Tatiana I.; Eichinger, Andreas; Deryugina, Elena I.; Strongin, Alex Y.

In: Journal of Biological Chemistry, Vol. 277, No. 11, 15.03.2002, p. 9318-9325.

Research output: Contribution to journalArticle

Rozanov, Dmitri ; Ghebrehiwet, Berhane ; Postnova, Tatiana I. ; Eichinger, Andreas ; Deryugina, Elena I. ; Strongin, Alex Y. / The hemopexin-like C-terminal domain of membrane type 1 matrix metalloproteinase regulates proteolysis of a multifunctional protein, gC1qR. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 11. pp. 9318-9325.
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