The helix-loop-helix Id-1 inhibits PSA expression in prostate cancer cells

Anne J. Zielinski, Sylvia Fong, Juanita Allison, Misako Kawahara, Jean Philippe Coppe, Heidi Feiler, Nancy M. Lee, Pierre Yves Desprez

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The inhibitor of basic helix-loop-helix transcription factors, Id-1, is an important gene whose expression increases during prostate cancer progression and that upregulates proliferation, migration and invasion. We used microarray analysis to identify the downstream genes whose transcriptional expression is modulated by Id-1 protein. We compared gene expression in control LNCaP cells and Id-1-transduced LNCaP cells, which become significantly more aggressive after Id-1 overexpression, thus mimicking the high levels of Id-1 detected in metastatic cell lines. We used the Affy HTA U133A Expression Arrays with 45,000 probe sets representing more than 39,000 transcripts. We found that one of the most significantly downregulated genes on Id-1 expression was kallikrein 3 [also called prostate specific antigen (PSA)], the most commonly used biomarker of prostate cancer. Here, we show that the reduction in PSA mRNA and protein expression associated with high-grade prostate cancers, which generally express high levels of Id-1, could be the consequence of Id-1 overexpression.

Original languageEnglish (US)
Pages (from-to)2490-2496
Number of pages7
JournalInternational Journal of Cancer
Volume126
Issue number10
DOIs
StatePublished - May 15 2010
Externally publishedYes

Keywords

  • Androgen receptor
  • Prostate acid phosphatase
  • Prostate specific antigen
  • Transcriptional regulator

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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