The generation and characterization of an ovary-selective cDNA library

Mamoru Tanaka, Jon Hennebold, Kei Miyakoshi, Takahide Teranishi, Kazunori Ueno, Eli Y. Adashi

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    We have utilized the differential screening technique suppression subtractive hybridization to systematically isolate and clone genes that are expressed in an ovary-selective/specific manner. In total, 844 clones were sequenced and analyzed for homology to known genes using the basic local alignment tool. One hundred and fifty nine independent clones proved identical to previously-characterized genes whereas an additional 100 independent clones proved significantly homologous (but not identical) to previously-characterized genes. Yet 83 other independent clones did not display significant homology to previously-characterized genes now listed in the publicly-accessible non-redundant databases. As such, these latter genes were deemed novel. In this communication we focus on two such novel ovary-specific/hormonally-dependent genes, the full-length sequences of which were isolated using RACE technology. These ovary-selective genes may have significant implications for the understanding of ovarian function in molecular terms and for the development of innovative strategies for the promotion of fertility or its control.

    Original languageEnglish (US)
    Pages (from-to)67-69
    Number of pages3
    JournalMolecular and Cellular Endocrinology
    Volume202
    Issue number1-2
    DOIs
    StatePublished - Apr 28 2003

    Fingerprint

    Gene Library
    Ovary
    Genes
    Clone Cells
    Fertility
    Screening
    Databases
    Technology
    Communication

    Keywords

    • Novel gene
    • Ovary
    • Suppression subtractive hybridization

    ASJC Scopus subject areas

    • Endocrinology
    • Endocrinology, Diabetes and Metabolism

    Cite this

    The generation and characterization of an ovary-selective cDNA library. / Tanaka, Mamoru; Hennebold, Jon; Miyakoshi, Kei; Teranishi, Takahide; Ueno, Kazunori; Adashi, Eli Y.

    In: Molecular and Cellular Endocrinology, Vol. 202, No. 1-2, 28.04.2003, p. 67-69.

    Research output: Contribution to journalArticle

    Tanaka, Mamoru ; Hennebold, Jon ; Miyakoshi, Kei ; Teranishi, Takahide ; Ueno, Kazunori ; Adashi, Eli Y. / The generation and characterization of an ovary-selective cDNA library. In: Molecular and Cellular Endocrinology. 2003 ; Vol. 202, No. 1-2. pp. 67-69.
    @article{c217825088a348a78c062fa635cb2020,
    title = "The generation and characterization of an ovary-selective cDNA library",
    abstract = "We have utilized the differential screening technique suppression subtractive hybridization to systematically isolate and clone genes that are expressed in an ovary-selective/specific manner. In total, 844 clones were sequenced and analyzed for homology to known genes using the basic local alignment tool. One hundred and fifty nine independent clones proved identical to previously-characterized genes whereas an additional 100 independent clones proved significantly homologous (but not identical) to previously-characterized genes. Yet 83 other independent clones did not display significant homology to previously-characterized genes now listed in the publicly-accessible non-redundant databases. As such, these latter genes were deemed novel. In this communication we focus on two such novel ovary-specific/hormonally-dependent genes, the full-length sequences of which were isolated using RACE technology. These ovary-selective genes may have significant implications for the understanding of ovarian function in molecular terms and for the development of innovative strategies for the promotion of fertility or its control.",
    keywords = "Novel gene, Ovary, Suppression subtractive hybridization",
    author = "Mamoru Tanaka and Jon Hennebold and Kei Miyakoshi and Takahide Teranishi and Kazunori Ueno and Adashi, {Eli Y.}",
    year = "2003",
    month = "4",
    day = "28",
    doi = "10.1016/S0303-7207(03)00064-9",
    language = "English (US)",
    volume = "202",
    pages = "67--69",
    journal = "Molecular and Cellular Endocrinology",
    issn = "0303-7207",
    publisher = "Elsevier Ireland Ltd",
    number = "1-2",

    }

    TY - JOUR

    T1 - The generation and characterization of an ovary-selective cDNA library

    AU - Tanaka, Mamoru

    AU - Hennebold, Jon

    AU - Miyakoshi, Kei

    AU - Teranishi, Takahide

    AU - Ueno, Kazunori

    AU - Adashi, Eli Y.

    PY - 2003/4/28

    Y1 - 2003/4/28

    N2 - We have utilized the differential screening technique suppression subtractive hybridization to systematically isolate and clone genes that are expressed in an ovary-selective/specific manner. In total, 844 clones were sequenced and analyzed for homology to known genes using the basic local alignment tool. One hundred and fifty nine independent clones proved identical to previously-characterized genes whereas an additional 100 independent clones proved significantly homologous (but not identical) to previously-characterized genes. Yet 83 other independent clones did not display significant homology to previously-characterized genes now listed in the publicly-accessible non-redundant databases. As such, these latter genes were deemed novel. In this communication we focus on two such novel ovary-specific/hormonally-dependent genes, the full-length sequences of which were isolated using RACE technology. These ovary-selective genes may have significant implications for the understanding of ovarian function in molecular terms and for the development of innovative strategies for the promotion of fertility or its control.

    AB - We have utilized the differential screening technique suppression subtractive hybridization to systematically isolate and clone genes that are expressed in an ovary-selective/specific manner. In total, 844 clones were sequenced and analyzed for homology to known genes using the basic local alignment tool. One hundred and fifty nine independent clones proved identical to previously-characterized genes whereas an additional 100 independent clones proved significantly homologous (but not identical) to previously-characterized genes. Yet 83 other independent clones did not display significant homology to previously-characterized genes now listed in the publicly-accessible non-redundant databases. As such, these latter genes were deemed novel. In this communication we focus on two such novel ovary-specific/hormonally-dependent genes, the full-length sequences of which were isolated using RACE technology. These ovary-selective genes may have significant implications for the understanding of ovarian function in molecular terms and for the development of innovative strategies for the promotion of fertility or its control.

    KW - Novel gene

    KW - Ovary

    KW - Suppression subtractive hybridization

    UR - http://www.scopus.com/inward/record.url?scp=0038288758&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0038288758&partnerID=8YFLogxK

    U2 - 10.1016/S0303-7207(03)00064-9

    DO - 10.1016/S0303-7207(03)00064-9

    M3 - Article

    VL - 202

    SP - 67

    EP - 69

    JO - Molecular and Cellular Endocrinology

    JF - Molecular and Cellular Endocrinology

    SN - 0303-7207

    IS - 1-2

    ER -