The gene for the axonal cell adhesion molecule TAX-1 is amplified and aberrantly expressed in malignant gliomas

David S. Rickman; Rachana Tyagi; Xiao Xiang Zhu; Miroslav P. Bobek; Suzan Song; Mila Blaivas; David E. Misek; Mark A. Israel; David M. Kurnit; Donald A. Ross; Phillip E. Kish; Samir M. Hanash

The gene for the axonal cell adhesion molecule TAX-1 is amplified and aberrantly expressed in malignant gliomas

David S. Rickman, Rachana Tyagi, Xiao Xiang Zhu, Miroslav P. Bobek, Suzan Song, Mila Blaivas, David E. Misek, Mark A. Israel, David M. Kurnit, Donald A. Ross, Phillip E. Kish, Samir M. Hanash

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

The human TAX-1 gene encodes a M_r 135,000 glycoprotein that is transiently expressed on the surface of a subset of neurons during development and is involved in neurite outgrowth. The TAX-1 gene has been mapped to a region on chromosome 1 that has been implicated in microcephaly and the Van der Woude syndrome. Using restriction landmark genome scanning to search for amplified genes in gliomas, we found TAX-1 to be amplified in 2 high-grade gliomas among a group of 26 gliomas investigated. Real-time reverse transcription-quantitative PCR analysis detected high levels of TAX-1 mRNA in glial tumors, even in the absence of TAX-1 gene amplification. Immunohistochemical analysis revealed abundant levels of TAX-1 in neoplastic glial cells of glioblastoma multiforme tumors. Because glial tumors are highly invasive and in view of the role of TAX-1 in neurite outgrowth, we investigated the potential role of TAX-1 in glioma cell migration. Using an in vitro assay, we found that the migration of glioma tumor cells is profoundly reduced in the presence of either an anti-TAX-1 antibody or a TAX-1 antisense oligonucleotide. Our findings suggest that TAX-1 plays a role in glial tumorigenesis and may provide a potential target for therapeutic intervention.

Original language	English (US)
Pages (from-to)	2162-2168
Number of pages	7
Journal	Cancer Research
Volume	61
Issue number	5
State	Published - Mar 1 2001
Externally published	Yes

ASJC Scopus subject areas

Oncology
Cancer Research

Cite this

@article{c02106ca01c048188707f962eb7766bb,

title = "The gene for the axonal cell adhesion molecule TAX-1 is amplified and aberrantly expressed in malignant gliomas",

abstract = "The human TAX-1 gene encodes a Mr 135,000 glycoprotein that is transiently expressed on the surface of a subset of neurons during development and is involved in neurite outgrowth. The TAX-1 gene has been mapped to a region on chromosome 1 that has been implicated in microcephaly and the Van der Woude syndrome. Using restriction landmark genome scanning to search for amplified genes in gliomas, we found TAX-1 to be amplified in 2 high-grade gliomas among a group of 26 gliomas investigated. Real-time reverse transcription-quantitative PCR analysis detected high levels of TAX-1 mRNA in glial tumors, even in the absence of TAX-1 gene amplification. Immunohistochemical analysis revealed abundant levels of TAX-1 in neoplastic glial cells of glioblastoma multiforme tumors. Because glial tumors are highly invasive and in view of the role of TAX-1 in neurite outgrowth, we investigated the potential role of TAX-1 in glioma cell migration. Using an in vitro assay, we found that the migration of glioma tumor cells is profoundly reduced in the presence of either an anti-TAX-1 antibody or a TAX-1 antisense oligonucleotide. Our findings suggest that TAX-1 plays a role in glial tumorigenesis and may provide a potential target for therapeutic intervention.",

author = "Rickman, {David S.} and Rachana Tyagi and Zhu, {Xiao Xiang} and Bobek, {Miroslav P.} and Suzan Song and Mila Blaivas and Misek, {David E.} and Israel, {Mark A.} and Kurnit, {David M.} and Ross, {Donald A.} and Kish, {Phillip E.} and Hanash, {Samir M.}",

year = "2001",

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language = "English (US)",

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T1 - The gene for the axonal cell adhesion molecule TAX-1 is amplified and aberrantly expressed in malignant gliomas

AU - Rickman, David S.

AU - Tyagi, Rachana

AU - Zhu, Xiao Xiang

AU - Bobek, Miroslav P.

AU - Song, Suzan

AU - Blaivas, Mila

AU - Misek, David E.

AU - Israel, Mark A.

AU - Kurnit, David M.

AU - Ross, Donald A.

AU - Kish, Phillip E.

AU - Hanash, Samir M.

PY - 2001/3/1

Y1 - 2001/3/1

N2 - The human TAX-1 gene encodes a Mr 135,000 glycoprotein that is transiently expressed on the surface of a subset of neurons during development and is involved in neurite outgrowth. The TAX-1 gene has been mapped to a region on chromosome 1 that has been implicated in microcephaly and the Van der Woude syndrome. Using restriction landmark genome scanning to search for amplified genes in gliomas, we found TAX-1 to be amplified in 2 high-grade gliomas among a group of 26 gliomas investigated. Real-time reverse transcription-quantitative PCR analysis detected high levels of TAX-1 mRNA in glial tumors, even in the absence of TAX-1 gene amplification. Immunohistochemical analysis revealed abundant levels of TAX-1 in neoplastic glial cells of glioblastoma multiforme tumors. Because glial tumors are highly invasive and in view of the role of TAX-1 in neurite outgrowth, we investigated the potential role of TAX-1 in glioma cell migration. Using an in vitro assay, we found that the migration of glioma tumor cells is profoundly reduced in the presence of either an anti-TAX-1 antibody or a TAX-1 antisense oligonucleotide. Our findings suggest that TAX-1 plays a role in glial tumorigenesis and may provide a potential target for therapeutic intervention.

AB - The human TAX-1 gene encodes a Mr 135,000 glycoprotein that is transiently expressed on the surface of a subset of neurons during development and is involved in neurite outgrowth. The TAX-1 gene has been mapped to a region on chromosome 1 that has been implicated in microcephaly and the Van der Woude syndrome. Using restriction landmark genome scanning to search for amplified genes in gliomas, we found TAX-1 to be amplified in 2 high-grade gliomas among a group of 26 gliomas investigated. Real-time reverse transcription-quantitative PCR analysis detected high levels of TAX-1 mRNA in glial tumors, even in the absence of TAX-1 gene amplification. Immunohistochemical analysis revealed abundant levels of TAX-1 in neoplastic glial cells of glioblastoma multiforme tumors. Because glial tumors are highly invasive and in view of the role of TAX-1 in neurite outgrowth, we investigated the potential role of TAX-1 in glioma cell migration. Using an in vitro assay, we found that the migration of glioma tumor cells is profoundly reduced in the presence of either an anti-TAX-1 antibody or a TAX-1 antisense oligonucleotide. Our findings suggest that TAX-1 plays a role in glial tumorigenesis and may provide a potential target for therapeutic intervention.

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The gene for the axonal cell adhesion molecule TAX-1 is amplified and aberrantly expressed in malignant gliomas

Abstract

ASJC Scopus subject areas

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