The gene for the axonal cell adhesion molecule TAX-1 is amplified and aberrantly expressed in malignant gliomas

David S. Rickman, Rachana Tyagi, Xiao Xiang Zhu, Miroslav P. Bobek, Suzan Song, Mila Blaivas, David E. Misek, Mark A. Israel, David M. Kurnit, Donald A. Ross, Phillip E. Kish, Samir M. Hanash

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

The human TAX-1 gene encodes a Mr 135,000 glycoprotein that is transiently expressed on the surface of a subset of neurons during development and is involved in neurite outgrowth. The TAX-1 gene has been mapped to a region on chromosome 1 that has been implicated in microcephaly and the Van der Woude syndrome. Using restriction landmark genome scanning to search for amplified genes in gliomas, we found TAX-1 to be amplified in 2 high-grade gliomas among a group of 26 gliomas investigated. Real-time reverse transcription-quantitative PCR analysis detected high levels of TAX-1 mRNA in glial tumors, even in the absence of TAX-1 gene amplification. Immunohistochemical analysis revealed abundant levels of TAX-1 in neoplastic glial cells of glioblastoma multiforme tumors. Because glial tumors are highly invasive and in view of the role of TAX-1 in neurite outgrowth, we investigated the potential role of TAX-1 in glioma cell migration. Using an in vitro assay, we found that the migration of glioma tumor cells is profoundly reduced in the presence of either an anti-TAX-1 antibody or a TAX-1 antisense oligonucleotide. Our findings suggest that TAX-1 plays a role in glial tumorigenesis and may provide a potential target for therapeutic intervention.

Original languageEnglish (US)
Pages (from-to)2162-2168
Number of pages7
JournalCancer Research
Volume61
Issue number5
StatePublished - Mar 1 2001

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Rickman, D. S., Tyagi, R., Zhu, X. X., Bobek, M. P., Song, S., Blaivas, M., Misek, D. E., Israel, M. A., Kurnit, D. M., Ross, D. A., Kish, P. E., & Hanash, S. M. (2001). The gene for the axonal cell adhesion molecule TAX-1 is amplified and aberrantly expressed in malignant gliomas. Cancer Research, 61(5), 2162-2168.