@article{c8911654d11b4d768806a2e36483d7d7,
title = "The functional diversity of the neuronal nicotinic acetylcholine receptors is increased by a novel subunit: β4",
abstract = "A new nicotinic acetylcholine receptor (nAChR) subunit, β4 was identified by screening a rat genomic library. In situ hybridization histochemistry revealed expression of the β4 gene in the medial habenula of adult rat brains. The primary structure of this subunit was deduced from a cDNA clone isolated from a PC12 cDNA library. Functional nAChRs were detected in Xenopus oocytes injected in pairwise combinations with in vitro synthesized RNAs encoding β4 and either the α2, 0, or α4 subunit. Unlike the a3β2 receptor, the α3β4 receptor is not blocked by bungarotoxin 3.1, indicating that the β subunit can affect the sensitivity of neuronal nAChRs to this toxin. These results extend the functional diversity of nicotinic receptors in the nervous system.",
author = "Duvoisin, {Robert M.} and Deneris, {Evan S.} and Jim Patrick and Steve Heinemann",
note = "Funding Information: We thank Anne O{\textquoteright}Shea-Greenfield and Karin Doebeli for preoaring the Xenopus oocytes. We are grateful to Dr. John Connolly for help with the electrophysiology experiments and Dr. Scott Rogers for the gift of 3.1 Bgt. We thank the above as well as Drs. Jim Boulter and Irm Hermans-Borgmeyer for helpful discussions and suggestions concerning the manuscript. We are indebted to Dr. Marc Ballivet for the gift of a rat genomic library and to Stratagene for providing us with their UniZAP kit before it was commercially available. This work was funded by grants from the National Institutes of Health (NS 11549 and NS 13546) and the Muscular Dystrophy Association. R. M. D. is supported by a Swiss National Science Foundation fellowship and was a postdoctoral fellow of the Muscular Dystrophy Association. E. S. D. is supported by a fellowship from the National Institutes Health.",
year = "1989",
month = oct,
doi = "10.1016/0896-6273(89)90207-9",
language = "English (US)",
volume = "3",
pages = "487--496",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "4",
}