The functional diversity of the neuronal nicotinic acetylcholine receptors is increased by a novel subunit

β4

Robert Duvoisin, Evan S. Deneris, Jim Patrick, Steve Heinemann

Research output: Contribution to journalArticle

231 Citations (Scopus)

Abstract

A new nicotinic acetylcholine receptor (nAChR) subunit, β4 was identified by screening a rat genomic library. In situ hybridization histochemistry revealed expression of the β4 gene in the medial habenula of adult rat brains. The primary structure of this subunit was deduced from a cDNA clone isolated from a PC12 cDNA library. Functional nAChRs were detected in Xenopus oocytes injected in pairwise combinations with in vitro synthesized RNAs encoding β4 and either the α2, 0, or α4 subunit. Unlike the a3β2 receptor, the α3β4 receptor is not blocked by bungarotoxin 3.1, indicating that the β subunit can affect the sensitivity of neuronal nAChRs to this toxin. These results extend the functional diversity of nicotinic receptors in the nervous system.

Original languageEnglish (US)
Pages (from-to)487-496
Number of pages10
JournalNeuron
Volume3
Issue number4
DOIs
StatePublished - 1989
Externally publishedYes

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Nicotinic Receptors
Habenula
Bungarotoxins
Genomic Library
Xenopus
Gene Library
Nervous System
Oocytes
In Situ Hybridization
Complementary DNA
Clone Cells
RNA
Gene Expression
Brain
In Vitro Techniques

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The functional diversity of the neuronal nicotinic acetylcholine receptors is increased by a novel subunit : β4. / Duvoisin, Robert; Deneris, Evan S.; Patrick, Jim; Heinemann, Steve.

In: Neuron, Vol. 3, No. 4, 1989, p. 487-496.

Research output: Contribution to journalArticle

Duvoisin, Robert ; Deneris, Evan S. ; Patrick, Jim ; Heinemann, Steve. / The functional diversity of the neuronal nicotinic acetylcholine receptors is increased by a novel subunit : β4. In: Neuron. 1989 ; Vol. 3, No. 4. pp. 487-496.
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