The FSH β-subunit promoter directs the expression of Herpes simplex virus type 1 thymidine kinase to the testis of transgenic mice

Merja A. Markkula, Tuula M. Hämäläinen, Fuping Zhang, Kyoon E. Kim, Richard Maurer, Ilpo T. Huhtaniemi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The bovine FSH β-subunit promoter (2.3 kb) was coupled to the coding sequence of the Herpes simplex virus type 1 thymidine kinase (HSV-tk) gene and introduced into mouse embryos. A full-length tk transcript was found in the pituitary and testis. In the testis an additional truncated version of tk mRNA was also expressed. Two sets of primer extension fragments were identified, one corresponding to transcription initiation at or near the cap site of the FSH-β gene, the other to transcription initiation within the tk gene. Furthermore, the latter, shorter transcript contained a 227 bp deletion. Only the long transcript was translated into immunoreactive tk in the later stages of developing spermatids. The tk protein was also functional in the testes, since spermatogenesis was either arrested or the germinal epithelium almost completely destroyed in transgenic males treated with the antiherpetic agent. If the FSH-β-HSV-tk transgene also functions correspondingly in the pituitary, these mice will provide a useful model for studies on FSH.

Original languageEnglish (US)
Pages (from-to)25-36
Number of pages12
JournalMolecular and Cellular Endocrinology
Volume96
Issue number1-2
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Thymidine Kinase
Human Herpesvirus 1
Viruses
Transgenic Mice
Testis
Genes
Transcription
Spermatids
Spermatogenesis
Transgenes
Embryonic Structures
Epithelium
Messenger RNA
thymidine kinase 1
Proteins

Keywords

  • Ablation
  • FSHβ
  • Herpes simplex virus thymidine kinase
  • Testis
  • Transgenic mice

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

The FSH β-subunit promoter directs the expression of Herpes simplex virus type 1 thymidine kinase to the testis of transgenic mice. / Markkula, Merja A.; Hämäläinen, Tuula M.; Zhang, Fuping; Kim, Kyoon E.; Maurer, Richard; Huhtaniemi, Ilpo T.

In: Molecular and Cellular Endocrinology, Vol. 96, No. 1-2, 1993, p. 25-36.

Research output: Contribution to journalArticle

Markkula, Merja A. ; Hämäläinen, Tuula M. ; Zhang, Fuping ; Kim, Kyoon E. ; Maurer, Richard ; Huhtaniemi, Ilpo T. / The FSH β-subunit promoter directs the expression of Herpes simplex virus type 1 thymidine kinase to the testis of transgenic mice. In: Molecular and Cellular Endocrinology. 1993 ; Vol. 96, No. 1-2. pp. 25-36.
@article{54efff8f36ca4760a5ee9388c72c1d19,
title = "The FSH β-subunit promoter directs the expression of Herpes simplex virus type 1 thymidine kinase to the testis of transgenic mice",
abstract = "The bovine FSH β-subunit promoter (2.3 kb) was coupled to the coding sequence of the Herpes simplex virus type 1 thymidine kinase (HSV-tk) gene and introduced into mouse embryos. A full-length tk transcript was found in the pituitary and testis. In the testis an additional truncated version of tk mRNA was also expressed. Two sets of primer extension fragments were identified, one corresponding to transcription initiation at or near the cap site of the FSH-β gene, the other to transcription initiation within the tk gene. Furthermore, the latter, shorter transcript contained a 227 bp deletion. Only the long transcript was translated into immunoreactive tk in the later stages of developing spermatids. The tk protein was also functional in the testes, since spermatogenesis was either arrested or the germinal epithelium almost completely destroyed in transgenic males treated with the antiherpetic agent. If the FSH-β-HSV-tk transgene also functions correspondingly in the pituitary, these mice will provide a useful model for studies on FSH.",
keywords = "Ablation, FSHβ, Herpes simplex virus thymidine kinase, Testis, Transgenic mice",
author = "Markkula, {Merja A.} and H{\"a}m{\"a}l{\"a}inen, {Tuula M.} and Fuping Zhang and Kim, {Kyoon E.} and Richard Maurer and Huhtaniemi, {Ilpo T.}",
year = "1993",
doi = "10.1016/0303-7207(93)90091-W",
language = "English (US)",
volume = "96",
pages = "25--36",
journal = "Molecular and Cellular Endocrinology",
issn = "0303-7207",
publisher = "Elsevier Ireland Ltd",
number = "1-2",

}

TY - JOUR

T1 - The FSH β-subunit promoter directs the expression of Herpes simplex virus type 1 thymidine kinase to the testis of transgenic mice

AU - Markkula, Merja A.

AU - Hämäläinen, Tuula M.

AU - Zhang, Fuping

AU - Kim, Kyoon E.

AU - Maurer, Richard

AU - Huhtaniemi, Ilpo T.

PY - 1993

Y1 - 1993

N2 - The bovine FSH β-subunit promoter (2.3 kb) was coupled to the coding sequence of the Herpes simplex virus type 1 thymidine kinase (HSV-tk) gene and introduced into mouse embryos. A full-length tk transcript was found in the pituitary and testis. In the testis an additional truncated version of tk mRNA was also expressed. Two sets of primer extension fragments were identified, one corresponding to transcription initiation at or near the cap site of the FSH-β gene, the other to transcription initiation within the tk gene. Furthermore, the latter, shorter transcript contained a 227 bp deletion. Only the long transcript was translated into immunoreactive tk in the later stages of developing spermatids. The tk protein was also functional in the testes, since spermatogenesis was either arrested or the germinal epithelium almost completely destroyed in transgenic males treated with the antiherpetic agent. If the FSH-β-HSV-tk transgene also functions correspondingly in the pituitary, these mice will provide a useful model for studies on FSH.

AB - The bovine FSH β-subunit promoter (2.3 kb) was coupled to the coding sequence of the Herpes simplex virus type 1 thymidine kinase (HSV-tk) gene and introduced into mouse embryos. A full-length tk transcript was found in the pituitary and testis. In the testis an additional truncated version of tk mRNA was also expressed. Two sets of primer extension fragments were identified, one corresponding to transcription initiation at or near the cap site of the FSH-β gene, the other to transcription initiation within the tk gene. Furthermore, the latter, shorter transcript contained a 227 bp deletion. Only the long transcript was translated into immunoreactive tk in the later stages of developing spermatids. The tk protein was also functional in the testes, since spermatogenesis was either arrested or the germinal epithelium almost completely destroyed in transgenic males treated with the antiherpetic agent. If the FSH-β-HSV-tk transgene also functions correspondingly in the pituitary, these mice will provide a useful model for studies on FSH.

KW - Ablation

KW - FSHβ

KW - Herpes simplex virus thymidine kinase

KW - Testis

KW - Transgenic mice

UR - http://www.scopus.com/inward/record.url?scp=0027520508&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027520508&partnerID=8YFLogxK

U2 - 10.1016/0303-7207(93)90091-W

DO - 10.1016/0303-7207(93)90091-W

M3 - Article

C2 - 8276135

AN - SCOPUS:0027520508

VL - 96

SP - 25

EP - 36

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

SN - 0303-7207

IS - 1-2

ER -