The Formation of Microthrombi in Parenchymal Microvessels after Traumatic Brain Injury Is Independent of Coagulation Factor XI

Susanne M. Schwarzmaier, Ciaran De Chaumont, Matilde Balbi, Nicole A. Terpolilli, Christoph Kleinschnitz, Andras Gruber, Nikolaus Plesnila

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Microthrombus formation and bleeding worsen the outcome after traumatic brain injury (TBI). The aim of the current study was to characterize these processes in the brain parenchyma after experimental TBI and to determine the involvement of coagulation factor XI (FXI). C57BL/6 mice (n = 101) and FXI-deficient mice (n = 15) were subjected to controlled cortical impact (CCI). Wild-type mice received an inhibitory antibody against FXI (14E11) or control immunoglobulin G 24 h before or 30 or 120 min after CCI. Cerebral microcirculation was visualized in vivo by 2-photon microscopy 2-3 h post-trauma and histopathological outcome was assessed after 24 h. TBI induced hemorrhage and microthrombus formation in the brain parenchyma (p < 0.001). Inhibition of FXI activation or FXI deficiency did not reduce cerebral thrombogenesis, lesion volume, or hemispheric swelling. However, it also did not increase intracranial hemorrhage. Formation of microthrombosis in the brain parenchyma after TBI is independent of the intrinsic coagulation cascade since it was not reduced by inhibition of FXI. However, since targeting FXI has well-established antithrombotic effects in humans and experimental animals, inhibition of FXI could represent a reasonable strategy for the prevention of deep venous thrombosis in immobilized patients with TBI.

Original languageEnglish (US)
Pages (from-to)1634-1644
Number of pages11
JournalJournal of Neurotrauma
Volume33
Issue number17
DOIs
StatePublished - Sep 1 2016

Fingerprint

Factor XI
Microvessels
Brain
Factor XI Deficiency
Hemorrhage
Intracranial Hemorrhages
Microcirculation
Traumatic Brain Injury
Inbred C57BL Mouse
Photons
Venous Thrombosis
Microscopy
Immunoglobulin G
Antibodies
Wounds and Injuries

Keywords

  • 2-photon microscopy
  • anticoagulation
  • in vivo
  • microthrombi
  • traumatic brain injury

ASJC Scopus subject areas

  • Clinical Neurology

Cite this

Schwarzmaier, S. M., De Chaumont, C., Balbi, M., Terpolilli, N. A., Kleinschnitz, C., Gruber, A., & Plesnila, N. (2016). The Formation of Microthrombi in Parenchymal Microvessels after Traumatic Brain Injury Is Independent of Coagulation Factor XI. Journal of Neurotrauma, 33(17), 1634-1644. https://doi.org/10.1089/neu.2015.4173

The Formation of Microthrombi in Parenchymal Microvessels after Traumatic Brain Injury Is Independent of Coagulation Factor XI. / Schwarzmaier, Susanne M.; De Chaumont, Ciaran; Balbi, Matilde; Terpolilli, Nicole A.; Kleinschnitz, Christoph; Gruber, Andras; Plesnila, Nikolaus.

In: Journal of Neurotrauma, Vol. 33, No. 17, 01.09.2016, p. 1634-1644.

Research output: Contribution to journalArticle

Schwarzmaier, SM, De Chaumont, C, Balbi, M, Terpolilli, NA, Kleinschnitz, C, Gruber, A & Plesnila, N 2016, 'The Formation of Microthrombi in Parenchymal Microvessels after Traumatic Brain Injury Is Independent of Coagulation Factor XI', Journal of Neurotrauma, vol. 33, no. 17, pp. 1634-1644. https://doi.org/10.1089/neu.2015.4173
Schwarzmaier, Susanne M. ; De Chaumont, Ciaran ; Balbi, Matilde ; Terpolilli, Nicole A. ; Kleinschnitz, Christoph ; Gruber, Andras ; Plesnila, Nikolaus. / The Formation of Microthrombi in Parenchymal Microvessels after Traumatic Brain Injury Is Independent of Coagulation Factor XI. In: Journal of Neurotrauma. 2016 ; Vol. 33, No. 17. pp. 1634-1644.
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