The fetal spinal cord does not regenerate after in utero transection in a large mammalian model

Claudia Meuli-Simmen, Martin Meuli, Grover M. Hutchins, Charles D. Yingling, Gregory Timmel, Michael R. Harrison, N. Scott Adzick

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

OBJECTIVE: Regeneration and functional recovery after spinal cord transection do not occur in mammalian animals and humans postnatally. The goal of this study was to test whether in utero transection of the fetal spinal cord is succeeded by anatomic healing and functional recovery. METHODS: In five sheep fetuses, at 60 days of gestation and 75 days of gestation (term = 150 d), the spinal cord was completely transected at T10. The animals were delivered near term by cesarean section for clinical evaluation, measurement of cortical somatosensory evoked potentials, and morphological assessment. RESULTS: The newborn lambs demonstrated sensory- motor paraplegia, were incontinent of urine and stool, and exhibited a spinally generated, ambulatory pattern of the hindlimbs. No cortical somatosensory evoked potentials could be recorded in response to posterior tibial nerve stimulation, although potentials from the ulnar nerve, which enters the cord rostral to the lesion, were normal in all animals. Histologically, no neuronal connections across the transection site were identified. The cord proximal to the lesion was grossly normal, whereas distal to the transection, it appeared slightly smaller but with the cytoarchitecture preserved. CONCLUSIONS: Unlike in lower vertebrate and avian species, the fetal ovine spinal cord has no detectable spontaneous regenerative capabilities when transected during midgestation. Gap formation after transection, secondary posttraumatic cell death, and missing guiding channels for sprouting axons may be factors involved in the absence of any regenerative response.

Original languageEnglish (US)
Pages (from-to)555-561
Number of pages7
JournalNeurosurgery
Volume39
Issue number3
StatePublished - Sep 1996
Externally publishedYes

Fingerprint

Spinal Cord
Somatosensory Evoked Potentials
Spinal Cord Injuries
Sheep
Pregnancy
Tibial Nerve
Ulnar Nerve
Paraplegia
Hindlimb
Cesarean Section
Axons
Vertebrates
Regeneration
Fetus
Cell Death
Urine

Keywords

  • Axonal sprouting
  • Fetus
  • Neural regeneration
  • Spinal cord

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery

Cite this

Meuli-Simmen, C., Meuli, M., Hutchins, G. M., Yingling, C. D., Timmel, G., Harrison, M. R., & Adzick, N. S. (1996). The fetal spinal cord does not regenerate after in utero transection in a large mammalian model. Neurosurgery, 39(3), 555-561.

The fetal spinal cord does not regenerate after in utero transection in a large mammalian model. / Meuli-Simmen, Claudia; Meuli, Martin; Hutchins, Grover M.; Yingling, Charles D.; Timmel, Gregory; Harrison, Michael R.; Adzick, N. Scott.

In: Neurosurgery, Vol. 39, No. 3, 09.1996, p. 555-561.

Research output: Contribution to journalArticle

Meuli-Simmen, C, Meuli, M, Hutchins, GM, Yingling, CD, Timmel, G, Harrison, MR & Adzick, NS 1996, 'The fetal spinal cord does not regenerate after in utero transection in a large mammalian model', Neurosurgery, vol. 39, no. 3, pp. 555-561.
Meuli-Simmen C, Meuli M, Hutchins GM, Yingling CD, Timmel G, Harrison MR et al. The fetal spinal cord does not regenerate after in utero transection in a large mammalian model. Neurosurgery. 1996 Sep;39(3):555-561.
Meuli-Simmen, Claudia ; Meuli, Martin ; Hutchins, Grover M. ; Yingling, Charles D. ; Timmel, Gregory ; Harrison, Michael R. ; Adzick, N. Scott. / The fetal spinal cord does not regenerate after in utero transection in a large mammalian model. In: Neurosurgery. 1996 ; Vol. 39, No. 3. pp. 555-561.
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