The fecal microbiota of Thai school-aged children associated with demographic factors and diet

Lucsame Gruneck, Eleni Gentekaki, Kongkiat Kespechara, Justin Denny, Thomas J. Sharpton, Lisa K. Marriott, Jackilen Shannon, Siam Popluechai

Research output: Contribution to journalArticlepeer-review

Abstract

Background. Birth delivery method and breastfeeding practices contribute to microbiota colonization. Other factors including diet and demographic factors structure the gut microbiome assembly and diversity through childhood development. The exploration of these factors, especially in Southeast Asian children, remains limited. Methods. We investigated the fecal microbiota of 127 school-aged children in Thailand using quantitative PCR (qPCR) to assess the influence of diet and demographic factors on the gut microbiota. Multivariate analysis (multiple factor analysis (MFA) and Partial Least Squares Discriminant Analysis (PLS-DA)) were used to link particular gut microbes to diet and demographic factors. Results. Diet and demographic factors were associated with variation among gut microbiota. The abundance of Gammaproteobacteria increased in children with infrequent intake of high fat foods. Obese children possessed a lower level of Firmicutes and Ruminococcus. Bifidobacterium was enriched in pre-teen aged children and detected at lower levels among formula-fed children. Prevotella was more abundant in children who were delivered vaginally. While ethnicity explained a small amount of variation in the gut microbiota, it nonetheless was found to be significantly associated with microbiome composition. Conclusions. Exogenous and demographic factors associate with, and possibly drive, the assembly of the gut microbiome of an understudied population of school-aged children in Thailand.

Original languageEnglish (US)
Article numbere13325
JournalPeerJ
Volume10
DOIs
StatePublished - Apr 2022

Keywords

  • Dietary behaviors
  • Fecal microbiota
  • Quantitative PCR
  • School-aged children
  • Thai

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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