The Ets-related transcription factor PU.1 immortalizes erythroblasts

Scott Schuetze, Paula E. Stenberg, David Kabat

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

In vivo studies of Friend virus erythroleukemia have implied that proviral integrations adjacent to the gene for the Ets-related transcription factor PU.1 may inhibit the commitment of erythroblasts to differentiate and cause their capability for indefinite transplantation (C. Spiro, B. Gliniak, and D. Kabat, J. Virol. 62:4129-4135, 1988; R. Paul, S. Schuetze, S. L. Kozak, C. Kozak, and D. Kabat, J. Virol. 65:464-467, 1991). To test this hypothesis, we ligated PU.1 cDNA into a retroviral vector and studied its effects on cultured cells. Infection of fibroblasts with PU.1-encoding retrovirus resulted in PU.1 synthesis followed by nuclear pyknosis, cell rounding, and degeneration. In contrast, in long-term bone marrow cultures, erythroblasts were efficiently and rapidly immortalized. The resulting cell lines were polyclonal populations that contained PU.1, were morphologically blast-like, required erythropoietin and bone marrow stromal cells for survival and proliferation, and spontaneously differentiated at low frequency to synthesize hemoglobin. After 9 months in culture, erythroblasts became stroma independent, and they then grew as clonal cell lines. We conclude that PU.1 perturbs the pathway(s) that controls potential for indefinite proliferation and that it can be used to generate permanent erythroblast cell lines.

Original languageEnglish (US)
Pages (from-to)5670-5678
Number of pages9
JournalMolecular and Cellular Biology
Volume13
Issue number9
StatePublished - Sep 1993

Fingerprint

Proto-Oncogene Proteins c-ets
Erythroblasts
Cell Line
Friend murine leukemia virus
Leukemia, Erythroblastic, Acute
Retroviridae
Erythropoietin
Mesenchymal Stromal Cells
Cultured Cells
Cell Survival
Hemoglobins
Complementary DNA
Fibroblasts
Transplantation
Bone Marrow
Cell Proliferation
proto-oncogene protein Spi-1
Infection
Population
Genes

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Schuetze, S., Stenberg, P. E., & Kabat, D. (1993). The Ets-related transcription factor PU.1 immortalizes erythroblasts. Molecular and Cellular Biology, 13(9), 5670-5678.

The Ets-related transcription factor PU.1 immortalizes erythroblasts. / Schuetze, Scott; Stenberg, Paula E.; Kabat, David.

In: Molecular and Cellular Biology, Vol. 13, No. 9, 09.1993, p. 5670-5678.

Research output: Contribution to journalArticle

Schuetze, S, Stenberg, PE & Kabat, D 1993, 'The Ets-related transcription factor PU.1 immortalizes erythroblasts', Molecular and Cellular Biology, vol. 13, no. 9, pp. 5670-5678.
Schuetze, Scott ; Stenberg, Paula E. ; Kabat, David. / The Ets-related transcription factor PU.1 immortalizes erythroblasts. In: Molecular and Cellular Biology. 1993 ; Vol. 13, No. 9. pp. 5670-5678.
@article{37739431da174f4b904ac96985b19199,
title = "The Ets-related transcription factor PU.1 immortalizes erythroblasts",
abstract = "In vivo studies of Friend virus erythroleukemia have implied that proviral integrations adjacent to the gene for the Ets-related transcription factor PU.1 may inhibit the commitment of erythroblasts to differentiate and cause their capability for indefinite transplantation (C. Spiro, B. Gliniak, and D. Kabat, J. Virol. 62:4129-4135, 1988; R. Paul, S. Schuetze, S. L. Kozak, C. Kozak, and D. Kabat, J. Virol. 65:464-467, 1991). To test this hypothesis, we ligated PU.1 cDNA into a retroviral vector and studied its effects on cultured cells. Infection of fibroblasts with PU.1-encoding retrovirus resulted in PU.1 synthesis followed by nuclear pyknosis, cell rounding, and degeneration. In contrast, in long-term bone marrow cultures, erythroblasts were efficiently and rapidly immortalized. The resulting cell lines were polyclonal populations that contained PU.1, were morphologically blast-like, required erythropoietin and bone marrow stromal cells for survival and proliferation, and spontaneously differentiated at low frequency to synthesize hemoglobin. After 9 months in culture, erythroblasts became stroma independent, and they then grew as clonal cell lines. We conclude that PU.1 perturbs the pathway(s) that controls potential for indefinite proliferation and that it can be used to generate permanent erythroblast cell lines.",
author = "Scott Schuetze and Stenberg, {Paula E.} and David Kabat",
year = "1993",
month = "9",
language = "English (US)",
volume = "13",
pages = "5670--5678",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "9",

}

TY - JOUR

T1 - The Ets-related transcription factor PU.1 immortalizes erythroblasts

AU - Schuetze, Scott

AU - Stenberg, Paula E.

AU - Kabat, David

PY - 1993/9

Y1 - 1993/9

N2 - In vivo studies of Friend virus erythroleukemia have implied that proviral integrations adjacent to the gene for the Ets-related transcription factor PU.1 may inhibit the commitment of erythroblasts to differentiate and cause their capability for indefinite transplantation (C. Spiro, B. Gliniak, and D. Kabat, J. Virol. 62:4129-4135, 1988; R. Paul, S. Schuetze, S. L. Kozak, C. Kozak, and D. Kabat, J. Virol. 65:464-467, 1991). To test this hypothesis, we ligated PU.1 cDNA into a retroviral vector and studied its effects on cultured cells. Infection of fibroblasts with PU.1-encoding retrovirus resulted in PU.1 synthesis followed by nuclear pyknosis, cell rounding, and degeneration. In contrast, in long-term bone marrow cultures, erythroblasts were efficiently and rapidly immortalized. The resulting cell lines were polyclonal populations that contained PU.1, were morphologically blast-like, required erythropoietin and bone marrow stromal cells for survival and proliferation, and spontaneously differentiated at low frequency to synthesize hemoglobin. After 9 months in culture, erythroblasts became stroma independent, and they then grew as clonal cell lines. We conclude that PU.1 perturbs the pathway(s) that controls potential for indefinite proliferation and that it can be used to generate permanent erythroblast cell lines.

AB - In vivo studies of Friend virus erythroleukemia have implied that proviral integrations adjacent to the gene for the Ets-related transcription factor PU.1 may inhibit the commitment of erythroblasts to differentiate and cause their capability for indefinite transplantation (C. Spiro, B. Gliniak, and D. Kabat, J. Virol. 62:4129-4135, 1988; R. Paul, S. Schuetze, S. L. Kozak, C. Kozak, and D. Kabat, J. Virol. 65:464-467, 1991). To test this hypothesis, we ligated PU.1 cDNA into a retroviral vector and studied its effects on cultured cells. Infection of fibroblasts with PU.1-encoding retrovirus resulted in PU.1 synthesis followed by nuclear pyknosis, cell rounding, and degeneration. In contrast, in long-term bone marrow cultures, erythroblasts were efficiently and rapidly immortalized. The resulting cell lines were polyclonal populations that contained PU.1, were morphologically blast-like, required erythropoietin and bone marrow stromal cells for survival and proliferation, and spontaneously differentiated at low frequency to synthesize hemoglobin. After 9 months in culture, erythroblasts became stroma independent, and they then grew as clonal cell lines. We conclude that PU.1 perturbs the pathway(s) that controls potential for indefinite proliferation and that it can be used to generate permanent erythroblast cell lines.

UR - http://www.scopus.com/inward/record.url?scp=0027317480&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027317480&partnerID=8YFLogxK

M3 - Article

C2 - 8355708

AN - SCOPUS:0027317480

VL - 13

SP - 5670

EP - 5678

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 9

ER -