The esterification in vitro of free cholesterol in human and rat plasma

John A. Glomset, Frank Parker, Margaret Tjaden, Robert H. Williams

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The transformation in vitro of the plasma free cholesterol first observed by Sperry has been further studied. The drop in free cholesterol occuring on the incubation of rat plasma has been shown to be due to esterification by fatty acids. Radioactive cholesterol incubated in vitro in rat or human plasma becomes incorporated into each of the cholesterol ester subfractions obtained by silicic acid column chromatography. Under similar conditions of incubation no incorporation of free fatty acids into any of the plasma ester fractions has been demonstrated. However, when [14C]linoleic acid-labeled lecithin is incubated in vitro with rat or human plasma, radioactive cholesterol linoleate is formed as well as 14C free fatty acid, triglycerides, mono- and diglycerides, and probably also cephalin. Similarly, when [14C]palmitic acid-labeled tripalmitin is incubated with rat or human plasma, [14C]cholesterol palmitate results as well as 14C-labeled free fatty acid, mono- and diglycerides, cephalin and lecithin. It is concluded that the preformed fatty acid esters of the plasma are the source of the fatty acids for the cholesterol esterification reaction in vitro.

Original languageEnglish (US)
Pages (from-to)398-406
Number of pages9
JournalBiochimica et Biophysica Acta - General Subjects
Volume58
Issue number3
StatePublished - Apr 23 1962
Externally publishedYes

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Esterification
Rats
Cholesterol
Plasma (human)
Plasmas
Nonesterified Fatty Acids
Phosphatidylethanolamines
Monoglycerides
Fatty Acids
Lecithins
Diglycerides
Linoleic Acid
Esters
Silicic Acid
Column chromatography
Palmitic Acid
Cholesterol Esters
Palmitates
In Vitro Techniques
Triglycerides

ASJC Scopus subject areas

  • Medicine(all)

Cite this

The esterification in vitro of free cholesterol in human and rat plasma. / Glomset, John A.; Parker, Frank; Tjaden, Margaret; Williams, Robert H.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 58, No. 3, 23.04.1962, p. 398-406.

Research output: Contribution to journalArticle

Glomset, John A. ; Parker, Frank ; Tjaden, Margaret ; Williams, Robert H. / The esterification in vitro of free cholesterol in human and rat plasma. In: Biochimica et Biophysica Acta - General Subjects. 1962 ; Vol. 58, No. 3. pp. 398-406.
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