The erythroid-specific protein cGATA-1 mediates distal enhancer activity through a specialized β-globin TATA box

T. C. Fong, Beverly Emerson

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Abstract

The erythroid-specific protein cGATA-1 regulates the chick β-globin gene through GATA sequences present at the canonical TATA location in the promoter as well as in the distal 3' enhancer. We have analyzed β-globin transcription in transfected erythroid cells and in erythroid extracts to determine whether cGATA-1 binding at -30 regulates promoter or enhancer activity. The interaction of both cGATA-1 and TFIID at different times with the -30 GATA site is required for efficient β-globin expression in vivo, and the GATA enhancer site can functionally replace the TATA element in the β- globin promoter. TFIID initiates transcription in vitro by complexing with adaptor proteins and displacing cGATA-1 from the -30 GATA site. Mutations that abolish TFIID binding to the -30 GATA box inactivate the promoter, whereas elimination of cGATA-1 binding to this site selectively diminishes enhancer-dependent transcription. We propose that interaction of cGATA-1 with the distal 3' enhancer and the specialized TATA box confers erythroid specificity to the initiation complex by mediating promoter-enhancer communication. Thus, one mechanism of action for tissue-specific proteins that recognizes noncanonical TATA motifs is to enable TFIID to be regulated by distal control elements. In this way, the initiation complex can be responsive to specific regulators that may not recognize a canonical TFIID- TATA structure.

Original languageEnglish (US)
Pages (from-to)521-532
Number of pages12
JournalGenes and Development
Volume6
Issue number4
DOIs
Publication statusPublished - Jan 1 1992
Externally publishedYes

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ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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