The enhancer domain of the human cytomegalovirus major immediate-early promoter determines cell type-specific expression in transgenic mice

John F. Baskar, Patricia P. Smith, Gajanan Nilaver, Ray A. Jupp, Stefan Hoffmann, Nancy J. Peffer, Daniel J. Tenney, Anamaris M. Colberg-Poley, Peter Ghazal, Jay Nelson

Research output: Contribution to journalArticle

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Abstract

The human cytomegalovirus (HCMV) major immediate-early promoter (MIEP) is one of the first promoters to activate upon infection. To examine HCMV MIEP tissue-specific expression, transgenic mice were established containing the lacZ gene regulated by the MIEP (nucleotides -670 to +54). In the transgenic mice, lacZ expression was demonstrated in 19 of 29 tissues tested by histochemical and immunochemical analyses. These tissues included brain, eye, spinal cord, esophagus, stomach, pancreas, kidney, bladder, testis, ovary, spleen, salivary gland, thymus, bone marrow, skin, cartilage, and cardiac, striated and smooth muscles. Although expression was observed in multiple organs, promoter activity was restricted to specific cell types. The cell types which demonstrated HCMV MIEP expression included retinal cells of the eye, ductile cells of the salivary gland, exocrine cells of the pancreas, mucosal cells of the stomach and intestine, neuronal cells of the brain, muscle fibers, thecal cells of the corpus luteum, and Leydig and sperm cells of the testis. These observations indicate that the HCMV MIEP is not a pan- specific promoter and that the majority of expressing tissues correlate with tissues naturally infected by the virus in the human host.

Original languageEnglish (US)
Pages (from-to)3207-3214
Number of pages8
JournalJournal of Virology
Volume70
Issue number5
StatePublished - 1996

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Human herpesvirus 5
Cytomegalovirus
Transgenic Mice
promoter regions
gene expression
mice
cells
Salivary Glands
salivary glands
Testis
pancreas
Stomach
testes
stomach
eyes
thecal cells
Exocrine Pancreas
Lac Operon
Striated Muscle
Leydig Cells

ASJC Scopus subject areas

  • Immunology

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The enhancer domain of the human cytomegalovirus major immediate-early promoter determines cell type-specific expression in transgenic mice. / Baskar, John F.; Smith, Patricia P.; Nilaver, Gajanan; Jupp, Ray A.; Hoffmann, Stefan; Peffer, Nancy J.; Tenney, Daniel J.; Colberg-Poley, Anamaris M.; Ghazal, Peter; Nelson, Jay.

In: Journal of Virology, Vol. 70, No. 5, 1996, p. 3207-3214.

Research output: Contribution to journalArticle

Baskar, JF, Smith, PP, Nilaver, G, Jupp, RA, Hoffmann, S, Peffer, NJ, Tenney, DJ, Colberg-Poley, AM, Ghazal, P & Nelson, J 1996, 'The enhancer domain of the human cytomegalovirus major immediate-early promoter determines cell type-specific expression in transgenic mice', Journal of Virology, vol. 70, no. 5, pp. 3207-3214.
Baskar, John F. ; Smith, Patricia P. ; Nilaver, Gajanan ; Jupp, Ray A. ; Hoffmann, Stefan ; Peffer, Nancy J. ; Tenney, Daniel J. ; Colberg-Poley, Anamaris M. ; Ghazal, Peter ; Nelson, Jay. / The enhancer domain of the human cytomegalovirus major immediate-early promoter determines cell type-specific expression in transgenic mice. In: Journal of Virology. 1996 ; Vol. 70, No. 5. pp. 3207-3214.
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