The electrochemical and fluorescence detection of nitric oxide in the cochlea and its increase following loud sound

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Abstract

A nitric oxide (NO)-selective sensor (tip diameter 30 μm) was inserted into the perilymph of the basal turn of the guinea pig cochlea. The basal level and stimulation-induced changes of NO were measured. The mean (±S.E.M.) basal level of NO was 273±42.9 nM. Following perilymphatic perfusion of the artificial perilymph containing NO synthase (NOS) substrate L-arginine (100 μM) combined with cofactor (6R)-5,6,7,8-tetrahydrobiopterin dihydrochloride (100 μM), a rapid and significant increase of NO to a mean concentration of 392±32.3 nM (PG-nitro-L-arginine methyl ester (100 μM). No change in the NO concentration was found following the perfusion of either artificial perilymph or NG-monomethyl-D-arginine (100 μM) solution employed as controls. Broadband noise exposure (3 h/day at 120 dBA SPL) for three consecutive days produced an increase in NO concentration to 618±60.7 nM (P

Original languageEnglish (US)
Pages (from-to)49-58
Number of pages10
JournalHearing Research
Volume164
Issue number1-2
DOIs
StatePublished - 2002

Fingerprint

Cochlea
Nitric Oxide
Fluorescence
Perilymph
Arginine
Perfusion
Nitric Oxide Synthase
Noise
Guinea Pigs

Keywords

  • Guinea pig
  • L-Arginine and (6R)-5,6,7,8-tetrahydrobiopterin dihydrochloride
  • N-Nitro-L-arginine methyl ester
  • Nitric oxide electrode
  • Noise exposure
  • Polarography
  • Reactive oxygen species

ASJC Scopus subject areas

  • Sensory Systems

Cite this

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title = "The electrochemical and fluorescence detection of nitric oxide in the cochlea and its increase following loud sound",
abstract = "A nitric oxide (NO)-selective sensor (tip diameter 30 μm) was inserted into the perilymph of the basal turn of the guinea pig cochlea. The basal level and stimulation-induced changes of NO were measured. The mean (±S.E.M.) basal level of NO was 273±42.9 nM. Following perilymphatic perfusion of the artificial perilymph containing NO synthase (NOS) substrate L-arginine (100 μM) combined with cofactor (6R)-5,6,7,8-tetrahydrobiopterin dihydrochloride (100 μM), a rapid and significant increase of NO to a mean concentration of 392±32.3 nM (PG-nitro-L-arginine methyl ester (100 μM). No change in the NO concentration was found following the perfusion of either artificial perilymph or NG-monomethyl-D-arginine (100 μM) solution employed as controls. Broadband noise exposure (3 h/day at 120 dBA SPL) for three consecutive days produced an increase in NO concentration to 618±60.7 nM (P",
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author = "Xiao Shi and Tianying Ren and Alfred Nuttall",
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AU - Shi, Xiao

AU - Ren, Tianying

AU - Nuttall, Alfred

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N2 - A nitric oxide (NO)-selective sensor (tip diameter 30 μm) was inserted into the perilymph of the basal turn of the guinea pig cochlea. The basal level and stimulation-induced changes of NO were measured. The mean (±S.E.M.) basal level of NO was 273±42.9 nM. Following perilymphatic perfusion of the artificial perilymph containing NO synthase (NOS) substrate L-arginine (100 μM) combined with cofactor (6R)-5,6,7,8-tetrahydrobiopterin dihydrochloride (100 μM), a rapid and significant increase of NO to a mean concentration of 392±32.3 nM (PG-nitro-L-arginine methyl ester (100 μM). No change in the NO concentration was found following the perfusion of either artificial perilymph or NG-monomethyl-D-arginine (100 μM) solution employed as controls. Broadband noise exposure (3 h/day at 120 dBA SPL) for three consecutive days produced an increase in NO concentration to 618±60.7 nM (P

AB - A nitric oxide (NO)-selective sensor (tip diameter 30 μm) was inserted into the perilymph of the basal turn of the guinea pig cochlea. The basal level and stimulation-induced changes of NO were measured. The mean (±S.E.M.) basal level of NO was 273±42.9 nM. Following perilymphatic perfusion of the artificial perilymph containing NO synthase (NOS) substrate L-arginine (100 μM) combined with cofactor (6R)-5,6,7,8-tetrahydrobiopterin dihydrochloride (100 μM), a rapid and significant increase of NO to a mean concentration of 392±32.3 nM (PG-nitro-L-arginine methyl ester (100 μM). No change in the NO concentration was found following the perfusion of either artificial perilymph or NG-monomethyl-D-arginine (100 μM) solution employed as controls. Broadband noise exposure (3 h/day at 120 dBA SPL) for three consecutive days produced an increase in NO concentration to 618±60.7 nM (P

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KW - Noise exposure

KW - Polarography

KW - Reactive oxygen species

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