The electrical response of cerebellar Purkinje neurons to simulated ischaemia

Martine Hamann, David J. Rossi, Claudia Mohr, Adriana L. Andrade, David Attwell

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Despite lacking N-methyl-D-aspartate receptors, cerebellar Purkinje cells are highly vulnerable to ischaemic insults, which lead them to die necrotically in an α-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA) receptor-dependent manner. To investigate the electrical events leading to this cell death, we whole-cell clamped Purkinje cells in cerebellar slices. Simulated ischaemia evoked an initial hyperpolarization of Purkinje cells by 8.5 mV, followed by a regenerative 'anoxic depolarization' (AD) to -14 mV. The AD was prevented by glutamate receptor blockers. In voltage-clamp mode, we used the cells' glutamate receptors to sense the rise of extracellular glutamate concentration induced by ischaemia, with GABAA and GABAB receptors blocked and Cs+ as the main pipette cation. Ischaemia induced a small (

Original languageEnglish (US)
Pages (from-to)2408-2420
Number of pages13
JournalBrain
Volume128
Issue number10
DOIs
Publication statusPublished - Oct 2005

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Keywords

  • Anoxia
  • Cerebellum
  • Exocytosis
  • Glutamate transporter
  • Ischaemia

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Hamann, M., Rossi, D. J., Mohr, C., Andrade, A. L., & Attwell, D. (2005). The electrical response of cerebellar Purkinje neurons to simulated ischaemia. Brain, 128(10), 2408-2420. https://doi.org/10.1093/brain/awh619