The effects of surgical and chemical lesions on striatal [3H]threo-(±)-methylphenidate binding

Correlation with [3H]dopamine uptake

Aaron Janowsky, Margaret M. Schweri, Paul Berger, Robert Long, Phil Skolnick, Steven M. Paul

Research output: Contribution to journalArticle

Abstract

The specific binding of [3H]threo-(±)-methylphenidate to membranes prepared from rat striatum was significantly reduced following either surgical lesions of the medial forebrain bundle or intracerebroventricular administration of 6-hydroxydopamine. The decrease in the density of [3H]threo-(±)-methylphenidate binding sites in striatum following chemical or surgical denervation was highly correlated with the decrease in [3H]dopamine uptake. In contrast, intracerebroventricular administration of 5,7-dihydroxytryptamine, AF64A, or chronic parenteral administration of reserpine did not alter either the number or apparent affinity of [3H]threo-(±)-methylphenidate binding sites. These data suggest that the specific binding sites for [3H]threo-(±)-methylphenidate in striatum are localized to dopaminergic nerve terminals, and may be associated with the dopamine transport complex.

Original languageEnglish (US)
Pages (from-to)187-191
Number of pages5
JournalEuropean Journal of Pharmacology
Volume108
Issue number2
DOIs
StatePublished - Jan 22 1985
Externally publishedYes

Fingerprint

Corpus Striatum
Methylphenidate
Dopamine
Binding Sites
5,7-Dihydroxytryptamine
Medial Forebrain Bundle
Oxidopamine
Reserpine
Denervation
Membranes

Keywords

  • [H]Dopamine uptake
  • [H]threo-(±)-Methylphenidate

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

The effects of surgical and chemical lesions on striatal [3H]threo-(±)-methylphenidate binding : Correlation with [3H]dopamine uptake. / Janowsky, Aaron; Schweri, Margaret M.; Berger, Paul; Long, Robert; Skolnick, Phil; Paul, Steven M.

In: European Journal of Pharmacology, Vol. 108, No. 2, 22.01.1985, p. 187-191.

Research output: Contribution to journalArticle

Janowsky, Aaron ; Schweri, Margaret M. ; Berger, Paul ; Long, Robert ; Skolnick, Phil ; Paul, Steven M. / The effects of surgical and chemical lesions on striatal [3H]threo-(±)-methylphenidate binding : Correlation with [3H]dopamine uptake. In: European Journal of Pharmacology. 1985 ; Vol. 108, No. 2. pp. 187-191.
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