The effects of surgical and chemical lesions on striatal [3H]threo-(±)-methylphenidate binding: Correlation with [3H]dopamine uptake

Aaron Janowsky, Margaret M. Schweri, Paul Berger, Robert Long, Phil Skolnick, Steven M. Paul

Research output: Contribution to journalArticle


The specific binding of [3H]threo-(±)-methylphenidate to membranes prepared from rat striatum was significantly reduced following either surgical lesions of the medial forebrain bundle or intracerebroventricular administration of 6-hydroxydopamine. The decrease in the density of [3H]threo-(±)-methylphenidate binding sites in striatum following chemical or surgical denervation was highly correlated with the decrease in [3H]dopamine uptake. In contrast, intracerebroventricular administration of 5,7-dihydroxytryptamine, AF64A, or chronic parenteral administration of reserpine did not alter either the number or apparent affinity of [3H]threo-(±)-methylphenidate binding sites. These data suggest that the specific binding sites for [3H]threo-(±)-methylphenidate in striatum are localized to dopaminergic nerve terminals, and may be associated with the dopamine transport complex.

Original languageEnglish (US)
Pages (from-to)187-191
Number of pages5
JournalEuropean Journal of Pharmacology
Issue number2
StatePublished - Jan 22 1985
Externally publishedYes



  • [H]Dopamine uptake
  • [H]threo-(±)-Methylphenidate

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this