The effects of chronic ethanol self-administration on hippocampal 5-HT1A receptors in monkeys

Elizabeth J. Burnett, Kathleen (Kathy) Grant, April T. Davenport, Scott E. Hemby, David P. Friedman

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Background: Chronic alcohol consumption reduces brain serotonin and alters the synaptic mechanisms involved in memory formation. Hippocampal 5-HT1A receptors modulate these mechanisms, but the neuroadaptive response of 5HT1A receptors to chronic alcohol self-administration is not well understood. Methods: Hippocampal tissue from monkeys that voluntarily self-administered ethanol for 12 months (n=9) and accompanying controls (n=8) were prepared for in vitro receptor autoradiography and laser capture microdissection. The 5-HT1A receptor antagonist, [3H]MPPF, and the agonist, [3H]8-OH-DPAT, were used to measure total and G-protein coupled 5-HT1A receptors respectively. The expression of the genes encoding the 5-HT1A receptor and its trafficking protein Yif1B was measured in microdissected dentate gyrus (DG) granule cells and CA1 pyramidal neurons. Results: An increase in G-protein coupled, but not total, receptors was observed in the posterior pyramidal cell layer of CA1 in ethanol drinkers compared to controls. Chronic ethanol self-administration was also associated with an up-regulation of total and G-protein coupled 5-HT1A receptors in the posterior DG polymorphic layer. Changes in receptor binding were not associated with concomitant changes in 5-HT1A receptor mRNA expression. Chronic ethanol self-administration was associated with a significant increase in Yif1B gene expression in posterior CA1 pyramidal neurons. Conclusions: Chronic, ethanol self-administration up-regulates hippocampal 5-HT1A receptor density in a region-specific manner that does not appear to be due to alterations at the level of transcription but instead may be due to increased receptor trafficking. Further exploration of the mechanisms mediating chronic ethanol-induced 5-HT1A receptor up-regulation and how hippocampal neurotransmission is altered is warranted.

    Original languageEnglish (US)
    Pages (from-to)135-142
    Number of pages8
    JournalDrug and Alcohol Dependence
    Volume136
    Issue number1
    DOIs
    StatePublished - Mar 1 2014

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    Receptor, Serotonin, 5-HT1A
    Self Administration
    Haplorhini
    Ethanol
    GTP-Binding Proteins
    Up-Regulation
    Pyramidal Cells
    Dentate Gyrus
    Neurons
    Microdissection
    Alcohols
    Serotonin 5-HT1 Receptor Antagonists
    Laser Capture Microdissection
    Gene Expression
    Hippocampal CA1 Region
    8-Hydroxy-2-(di-n-propylamino)tetralin
    Gene encoding
    Protein Transport
    Transcription
    Autoradiography

    Keywords

    • Heaving drinking
    • Hippocampus
    • Nonhuman primate
    • Serotonin
    • Yif1B

    ASJC Scopus subject areas

    • Psychiatry and Mental health
    • Toxicology
    • Pharmacology
    • Pharmacology (medical)

    Cite this

    The effects of chronic ethanol self-administration on hippocampal 5-HT1A receptors in monkeys. / Burnett, Elizabeth J.; Grant, Kathleen (Kathy); Davenport, April T.; Hemby, Scott E.; Friedman, David P.

    In: Drug and Alcohol Dependence, Vol. 136, No. 1, 01.03.2014, p. 135-142.

    Research output: Contribution to journalArticle

    Burnett, Elizabeth J. ; Grant, Kathleen (Kathy) ; Davenport, April T. ; Hemby, Scott E. ; Friedman, David P. / The effects of chronic ethanol self-administration on hippocampal 5-HT1A receptors in monkeys. In: Drug and Alcohol Dependence. 2014 ; Vol. 136, No. 1. pp. 135-142.
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    AU - Hemby, Scott E.

    AU - Friedman, David P.

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