The effect of wavelength on optical properties extracted from images of engineered tissue

David Levitz, Kevin G. Phillips, Lin An, Frederic Truffer, Ravikant Samatham, Niloy Choudhury, Monica Hinds, Stephen R. Hanson, Steven Jacques

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Optical imaging modalities such as confocal microscopy and optical coherence tomography (OCT) are emerging as appealing methods for non-destructive evaluation of engineered tissues. The information offered by such optical imaging methods depends on the wavelength vis-à-vis the optical scattering properties of the sample. These properties affect many factors critical to image analysis in a nonlinear and nontrivial manner. Thus, we sought to characterize the effect wavelength has on the optical properties collagen remodeled by cells at 3 common imaging wavelengths: 488, 633, and 1310 nm. To do this, we seeded smooth muscle cells (SMCs) in soluble collagen gels at a density of 1x106 cells/ml; similar acellular control constructs were also prepared. The constructs were allowed to remodel in the incubator for 5 days, and were examined at 24 and 120 hours by confocal imaging at 488 and 633 nm, and by OCT imaging at 1310 nm. From the confocal and OCT data, the attenuation and reflectivity were evaluated by fitting the data to a theoretical model that relates the tissue optical properties (scattering coefficient and anisotropy factor) and imaging conditions to the signal. In general, we found that at 1310 nm, the optical properties of the acellular control constructs had a lower reflectivity (higher anisotropy) than the SMC constructs. The difference in reflectivity between the SMC construct and acellular controls tended to decrease with wavelength, owing to a relative increase in reflectivity of acellular controls at lower wavelengths relative to the cellular constructs. Overall, the largest difference in optical properties occurred at 1310 nm. Taken together, the data show that the shift in optical properties of soluble collagen gels caused by cellular remodeling is nonlinearly wavelength dependent, and that this information should be considered when devising how to optimally characterize engineered tissues using optical imaging methods. @copy; 2009 SPIE.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7179
DOIs
StatePublished - 2009
EventOptics in Tissue Engineering and Regenerative Medicine III - San Jose, CA, United States
Duration: Jan 24 2009Jan 24 2009

Other

OtherOptics in Tissue Engineering and Regenerative Medicine III
CountryUnited States
CitySan Jose, CA
Period1/24/091/24/09

Fingerprint

Optical Properties
Optical properties
Wavelength
Smooth muscle Cells
Reflectivity
Tissue
Imaging techniques
smooth muscle
optical properties
muscle cells
Optical Coherence Tomography
Optical Imaging
Collagen
collagens
Imaging
Optical tomography
wavelengths
reflectance
Confocal
tomography

Keywords

  • Anisotropy
  • Cells
  • Collagen
  • Optical coherence tomography
  • Optical properties
  • Scattering
  • Tissue engineering

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Levitz, D., Phillips, K. G., An, L., Truffer, F., Samatham, R., Choudhury, N., ... Jacques, S. (2009). The effect of wavelength on optical properties extracted from images of engineered tissue. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7179). [717906] https://doi.org/10.1117/12.809653

The effect of wavelength on optical properties extracted from images of engineered tissue. / Levitz, David; Phillips, Kevin G.; An, Lin; Truffer, Frederic; Samatham, Ravikant; Choudhury, Niloy; Hinds, Monica; Hanson, Stephen R.; Jacques, Steven.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7179 2009. 717906.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Levitz, D, Phillips, KG, An, L, Truffer, F, Samatham, R, Choudhury, N, Hinds, M, Hanson, SR & Jacques, S 2009, The effect of wavelength on optical properties extracted from images of engineered tissue. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7179, 717906, Optics in Tissue Engineering and Regenerative Medicine III, San Jose, CA, United States, 1/24/09. https://doi.org/10.1117/12.809653
Levitz D, Phillips KG, An L, Truffer F, Samatham R, Choudhury N et al. The effect of wavelength on optical properties extracted from images of engineered tissue. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7179. 2009. 717906 https://doi.org/10.1117/12.809653
Levitz, David ; Phillips, Kevin G. ; An, Lin ; Truffer, Frederic ; Samatham, Ravikant ; Choudhury, Niloy ; Hinds, Monica ; Hanson, Stephen R. ; Jacques, Steven. / The effect of wavelength on optical properties extracted from images of engineered tissue. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7179 2009.
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abstract = "Optical imaging modalities such as confocal microscopy and optical coherence tomography (OCT) are emerging as appealing methods for non-destructive evaluation of engineered tissues. The information offered by such optical imaging methods depends on the wavelength vis-{\`a}-vis the optical scattering properties of the sample. These properties affect many factors critical to image analysis in a nonlinear and nontrivial manner. Thus, we sought to characterize the effect wavelength has on the optical properties collagen remodeled by cells at 3 common imaging wavelengths: 488, 633, and 1310 nm. To do this, we seeded smooth muscle cells (SMCs) in soluble collagen gels at a density of 1x106 cells/ml; similar acellular control constructs were also prepared. The constructs were allowed to remodel in the incubator for 5 days, and were examined at 24 and 120 hours by confocal imaging at 488 and 633 nm, and by OCT imaging at 1310 nm. From the confocal and OCT data, the attenuation and reflectivity were evaluated by fitting the data to a theoretical model that relates the tissue optical properties (scattering coefficient and anisotropy factor) and imaging conditions to the signal. In general, we found that at 1310 nm, the optical properties of the acellular control constructs had a lower reflectivity (higher anisotropy) than the SMC constructs. The difference in reflectivity between the SMC construct and acellular controls tended to decrease with wavelength, owing to a relative increase in reflectivity of acellular controls at lower wavelengths relative to the cellular constructs. Overall, the largest difference in optical properties occurred at 1310 nm. Taken together, the data show that the shift in optical properties of soluble collagen gels caused by cellular remodeling is nonlinearly wavelength dependent, and that this information should be considered when devising how to optimally characterize engineered tissues using optical imaging methods. @copy; 2009 SPIE.",
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