The effect of short moderate stress on the midbrain corticotropin-releasing factor system in a macaque model of functional hypothalamic amenorrhea

Cynthia Bethea, Kenny Phu, Arubala Reddy, Judy L. Cameron

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    Objective To study the effect of moderate stress on corticotropin-releasing factor (CRF) components in the serotonergic midbrain region in a monkey model of functional hypothalamic amenorrhea. Design After characterization of stress sensitivity, monkeys were moved to a novel room and given 20% less chow for 5 days before euthanasia. Setting Primate research center. Animal(s) Female cynomolgus macaques (Macaca fascicularis) characterized as highly stress resilient (HSR, n = 5), medium stress resilient (n = 4), or stress sensitive (SS, n = 4). Intervention(s) Five days of diet in a novel room with unfamiliar conspecifics. Main Outcome Measure(s) Density of CRF axons in the serotonergic dorsal raphe nucleus; the number of urocortin 1 (UCN1) cells; the density of UCN1 axons; the expression of CRF receptor 1 (CRF-R1) and CRF-R2 in the dorsal raphe nucleus. Result(s) The CRF innervation was higher in HSR than in SS animals; UCN1 cell number was higher in HSR than in SS animals and UCN1 axon bouton density was not different; all opposite of nonstressed animals. The CRF-R1 was not different between the sensitivity groups, but CRF-R2 was higher in HSR than in SS animals. The relative expression of CRF-R1 and CRF-R2 was similar to nonstressed animals. Conclusion(s) The HSR animals respond to stress with an increase in CRF delivery to serotonin neurons. With stress, UCN1 transport decreases in HSR animals. The CRF receptor expression was similar with or without stress. These changes may contribute to resilience in HSR animals.

    Original languageEnglish (US)
    JournalFertility and Sterility
    Volume100
    Issue number4
    DOIs
    StatePublished - Oct 2013

    Fingerprint

    Corticotropin-Releasing Hormone
    Amenorrhea
    Macaca
    Mesencephalon
    Urocortins
    Axons
    Haplorhini
    Corticotropin-Releasing Hormone Receptors
    Euthanasia
    Macaca fascicularis
    Primates
    Serotonin
    Cell Count
    Outcome Assessment (Health Care)
    Diet
    Neurons

    Keywords

    • amenorrhea
    • CRF
    • macaques
    • ovulation
    • resilience
    • serotonin
    • Stress

    ASJC Scopus subject areas

    • Obstetrics and Gynecology
    • Reproductive Medicine

    Cite this

    The effect of short moderate stress on the midbrain corticotropin-releasing factor system in a macaque model of functional hypothalamic amenorrhea. / Bethea, Cynthia; Phu, Kenny; Reddy, Arubala; Cameron, Judy L.

    In: Fertility and Sterility, Vol. 100, No. 4, 10.2013.

    Research output: Contribution to journalArticle

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    abstract = "Objective To study the effect of moderate stress on corticotropin-releasing factor (CRF) components in the serotonergic midbrain region in a monkey model of functional hypothalamic amenorrhea. Design After characterization of stress sensitivity, monkeys were moved to a novel room and given 20{\%} less chow for 5 days before euthanasia. Setting Primate research center. Animal(s) Female cynomolgus macaques (Macaca fascicularis) characterized as highly stress resilient (HSR, n = 5), medium stress resilient (n = 4), or stress sensitive (SS, n = 4). Intervention(s) Five days of diet in a novel room with unfamiliar conspecifics. Main Outcome Measure(s) Density of CRF axons in the serotonergic dorsal raphe nucleus; the number of urocortin 1 (UCN1) cells; the density of UCN1 axons; the expression of CRF receptor 1 (CRF-R1) and CRF-R2 in the dorsal raphe nucleus. Result(s) The CRF innervation was higher in HSR than in SS animals; UCN1 cell number was higher in HSR than in SS animals and UCN1 axon bouton density was not different; all opposite of nonstressed animals. The CRF-R1 was not different between the sensitivity groups, but CRF-R2 was higher in HSR than in SS animals. The relative expression of CRF-R1 and CRF-R2 was similar to nonstressed animals. Conclusion(s) The HSR animals respond to stress with an increase in CRF delivery to serotonin neurons. With stress, UCN1 transport decreases in HSR animals. The CRF receptor expression was similar with or without stress. These changes may contribute to resilience in HSR animals.",
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    AB - Objective To study the effect of moderate stress on corticotropin-releasing factor (CRF) components in the serotonergic midbrain region in a monkey model of functional hypothalamic amenorrhea. Design After characterization of stress sensitivity, monkeys were moved to a novel room and given 20% less chow for 5 days before euthanasia. Setting Primate research center. Animal(s) Female cynomolgus macaques (Macaca fascicularis) characterized as highly stress resilient (HSR, n = 5), medium stress resilient (n = 4), or stress sensitive (SS, n = 4). Intervention(s) Five days of diet in a novel room with unfamiliar conspecifics. Main Outcome Measure(s) Density of CRF axons in the serotonergic dorsal raphe nucleus; the number of urocortin 1 (UCN1) cells; the density of UCN1 axons; the expression of CRF receptor 1 (CRF-R1) and CRF-R2 in the dorsal raphe nucleus. Result(s) The CRF innervation was higher in HSR than in SS animals; UCN1 cell number was higher in HSR than in SS animals and UCN1 axon bouton density was not different; all opposite of nonstressed animals. The CRF-R1 was not different between the sensitivity groups, but CRF-R2 was higher in HSR than in SS animals. The relative expression of CRF-R1 and CRF-R2 was similar to nonstressed animals. Conclusion(s) The HSR animals respond to stress with an increase in CRF delivery to serotonin neurons. With stress, UCN1 transport decreases in HSR animals. The CRF receptor expression was similar with or without stress. These changes may contribute to resilience in HSR animals.

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    KW - serotonin

    KW - Stress

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