The effect of photodynamic action on corneal cells in tissue culture

Moshe Lahav, Daniel Albert, Nancy L. Robinson, David K. Dueker

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The effect of photodynamic inactivation on corneal epithelial, stromal and endothelial cells was investigated in tissue cultures. In contrast to reports regarding in vivo studies, the in vitro treatment caused significant degeneration and growth inhibition of stromal corneal cells and less marked changes of epithelial corneal cells in tissue cultures. Stationary endothelial cells appeared least effected. The neutral red was seen in the intracytoplasmic granules and in time localized mostly in the perinuclear area, and to a lesser extent within the nucleus. The in vitro study of actively growing corneal cells appears to be a sensitive indicator of cell damage following photoinactivation.

Original languageEnglish (US)
Pages (from-to)571-583
Number of pages13
JournalExperimental Eye Research
Volume20
Issue number6
DOIs
StatePublished - Jan 1 1975
Externally publishedYes

Fingerprint

Stromal Cells
Endothelial Cells
Epithelial Cells
Neutral Red
Growth
In Vitro Techniques

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

The effect of photodynamic action on corneal cells in tissue culture. / Lahav, Moshe; Albert, Daniel; Robinson, Nancy L.; Dueker, David K.

In: Experimental Eye Research, Vol. 20, No. 6, 01.01.1975, p. 571-583.

Research output: Contribution to journalArticle

Lahav, Moshe ; Albert, Daniel ; Robinson, Nancy L. ; Dueker, David K. / The effect of photodynamic action on corneal cells in tissue culture. In: Experimental Eye Research. 1975 ; Vol. 20, No. 6. pp. 571-583.
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