The effect of partial zona dissection on the fertilizing capacity of chronically obstructed mouse caput epididymal sperm

Douglas M. Dewire, Nina Davis, Kurt F. Miller, Nicholas T. Stowe, Anthony J. Thomas

Research output: Contribution to journalArticle

Abstract

Purpose: Having observed that sperm from the chronically obstructed caput epididymis fertilize poorly in vitro, we investigated the effect of partial zona dissection (PZD) on fertilization in a murine model of unilateral proximal epididymal obstruction. Cleavage rates were compared for zona-intact oocytes and PZD oocytes incubated with sperm from the following epididymal segments: the obstructed caput, the contralateral nonobstructed caput, the contralateral cauda, and a sperm-free preparation to control for parthenogenesis. Results: Unilateral epididymal obstruction resulted in significantly higher sperm counts in the obstructed caput compared to the nonobstructed caput, although there was no difference in motility. Cleavage rates for ova incubated with sperm from the obstructed caput and the nonobstructed caput were uniformly poor and did not differ significantly from those for the sperm-free controls. Cauda sperm fertilized significantly better than all other sperm groups (P

Original languageEnglish (US)
Pages (from-to)43-48
Number of pages6
JournalJournal of Assisted Reproduction and Genetics
Volume11
Issue number1
DOIs
StatePublished - Jan 1994
Externally publishedYes

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Herpes Zoster
Spermatozoa
Dissection
Oocytes
Parthenogenesis
Sperm Count
Epididymis
Fertilization
Ovum

Keywords

  • epididymis
  • in vitro fertilization
  • infertility
  • micromanipulation
  • spermatozoa

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology
  • Genetics
  • Developmental Biology

Cite this

The effect of partial zona dissection on the fertilizing capacity of chronically obstructed mouse caput epididymal sperm. / Dewire, Douglas M.; Davis, Nina; Miller, Kurt F.; Stowe, Nicholas T.; Thomas, Anthony J.

In: Journal of Assisted Reproduction and Genetics, Vol. 11, No. 1, 01.1994, p. 43-48.

Research output: Contribution to journalArticle

Dewire, Douglas M. ; Davis, Nina ; Miller, Kurt F. ; Stowe, Nicholas T. ; Thomas, Anthony J. / The effect of partial zona dissection on the fertilizing capacity of chronically obstructed mouse caput epididymal sperm. In: Journal of Assisted Reproduction and Genetics. 1994 ; Vol. 11, No. 1. pp. 43-48.
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