The effect of ketoconazole on steroidogenesis. II. Adrenocortical enzyme activity in vitro

B. D. Albertson, N. C. Maronian, K. L. Frederick, M. DiMattina, P. Feuillan, J. F. Dunn, Donald (Lynn) Loriaux

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The activity of five adrenocortical steroidogenic enzymes, 3β-hydroxysteroid dehydrogenase/isomerase (3β-HSD), 17-hydroxylase (17-OHase) 17,20 desmolase (17,20D), 21-hydroxylase (21-OHase) and 11-hydroxylase (11-OHase), were measured in vitro in purified mitochondria or microsomes from rhesus monkey (Macaca mulata) and human adrenal tissue in the presence and absence of graded concentrations of ketoconazole. Rhesus 3β-HSD activity was unaffected by ketoconazole at concentrations up to 5000 uM. However, human adrenal 3β-HSD was inhibited by approximately 40% (p

Original languageEnglish (US)
Pages (from-to)27-34
Number of pages8
JournalResearch Communications in Chemical Pathology and Pharmacology
Volume61
Issue number1
StatePublished - 1988
Externally publishedYes

Fingerprint

3-Hydroxysteroid Dehydrogenases
Isomerases
Ketoconazole
Enzyme activity
Mixed Function Oxygenases
Enzymes
Steroid 17-alpha-Hydroxylase
Steroid 21-Hydroxylase
Mitochondria
Macaca
Microsomes
Macaca mulatta
Tissue
In Vitro Techniques

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Albertson, B. D., Maronian, N. C., Frederick, K. L., DiMattina, M., Feuillan, P., Dunn, J. F., & Loriaux, D. L. (1988). The effect of ketoconazole on steroidogenesis. II. Adrenocortical enzyme activity in vitro. Research Communications in Chemical Pathology and Pharmacology, 61(1), 27-34.

The effect of ketoconazole on steroidogenesis. II. Adrenocortical enzyme activity in vitro. / Albertson, B. D.; Maronian, N. C.; Frederick, K. L.; DiMattina, M.; Feuillan, P.; Dunn, J. F.; Loriaux, Donald (Lynn).

In: Research Communications in Chemical Pathology and Pharmacology, Vol. 61, No. 1, 1988, p. 27-34.

Research output: Contribution to journalArticle

Albertson, BD, Maronian, NC, Frederick, KL, DiMattina, M, Feuillan, P, Dunn, JF & Loriaux, DL 1988, 'The effect of ketoconazole on steroidogenesis. II. Adrenocortical enzyme activity in vitro', Research Communications in Chemical Pathology and Pharmacology, vol. 61, no. 1, pp. 27-34.
Albertson BD, Maronian NC, Frederick KL, DiMattina M, Feuillan P, Dunn JF et al. The effect of ketoconazole on steroidogenesis. II. Adrenocortical enzyme activity in vitro. Research Communications in Chemical Pathology and Pharmacology. 1988;61(1):27-34.
Albertson, B. D. ; Maronian, N. C. ; Frederick, K. L. ; DiMattina, M. ; Feuillan, P. ; Dunn, J. F. ; Loriaux, Donald (Lynn). / The effect of ketoconazole on steroidogenesis. II. Adrenocortical enzyme activity in vitro. In: Research Communications in Chemical Pathology and Pharmacology. 1988 ; Vol. 61, No. 1. pp. 27-34.
@article{69a264f544b644edb47ff4b3d38f4adc,
title = "The effect of ketoconazole on steroidogenesis. II. Adrenocortical enzyme activity in vitro",
abstract = "The activity of five adrenocortical steroidogenic enzymes, 3β-hydroxysteroid dehydrogenase/isomerase (3β-HSD), 17-hydroxylase (17-OHase) 17,20 desmolase (17,20D), 21-hydroxylase (21-OHase) and 11-hydroxylase (11-OHase), were measured in vitro in purified mitochondria or microsomes from rhesus monkey (Macaca mulata) and human adrenal tissue in the presence and absence of graded concentrations of ketoconazole. Rhesus 3β-HSD activity was unaffected by ketoconazole at concentrations up to 5000 uM. However, human adrenal 3β-HSD was inhibited by approximately 40{\%} (p",
author = "Albertson, {B. D.} and Maronian, {N. C.} and Frederick, {K. L.} and M. DiMattina and P. Feuillan and Dunn, {J. F.} and Loriaux, {Donald (Lynn)}",
year = "1988",
language = "English (US)",
volume = "61",
pages = "27--34",
journal = "Research Communications in Molecular Pathology and Pharmacology",
issn = "0034-5164",
publisher = "PJD Publications Ltd",
number = "1",

}

TY - JOUR

T1 - The effect of ketoconazole on steroidogenesis. II. Adrenocortical enzyme activity in vitro

AU - Albertson, B. D.

AU - Maronian, N. C.

AU - Frederick, K. L.

AU - DiMattina, M.

AU - Feuillan, P.

AU - Dunn, J. F.

AU - Loriaux, Donald (Lynn)

PY - 1988

Y1 - 1988

N2 - The activity of five adrenocortical steroidogenic enzymes, 3β-hydroxysteroid dehydrogenase/isomerase (3β-HSD), 17-hydroxylase (17-OHase) 17,20 desmolase (17,20D), 21-hydroxylase (21-OHase) and 11-hydroxylase (11-OHase), were measured in vitro in purified mitochondria or microsomes from rhesus monkey (Macaca mulata) and human adrenal tissue in the presence and absence of graded concentrations of ketoconazole. Rhesus 3β-HSD activity was unaffected by ketoconazole at concentrations up to 5000 uM. However, human adrenal 3β-HSD was inhibited by approximately 40% (p

AB - The activity of five adrenocortical steroidogenic enzymes, 3β-hydroxysteroid dehydrogenase/isomerase (3β-HSD), 17-hydroxylase (17-OHase) 17,20 desmolase (17,20D), 21-hydroxylase (21-OHase) and 11-hydroxylase (11-OHase), were measured in vitro in purified mitochondria or microsomes from rhesus monkey (Macaca mulata) and human adrenal tissue in the presence and absence of graded concentrations of ketoconazole. Rhesus 3β-HSD activity was unaffected by ketoconazole at concentrations up to 5000 uM. However, human adrenal 3β-HSD was inhibited by approximately 40% (p

UR - http://www.scopus.com/inward/record.url?scp=0023687804&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023687804&partnerID=8YFLogxK

M3 - Article

C2 - 3175341

AN - SCOPUS:0023687804

VL - 61

SP - 27

EP - 34

JO - Research Communications in Molecular Pathology and Pharmacology

JF - Research Communications in Molecular Pathology and Pharmacology

SN - 0034-5164

IS - 1

ER -