Abstract
The effect of insulin and glucose on fructose-2,6-P2 turnover has been examined using isolated rat hepatocytes. Insulin (>10-10M) increases the fructose-2,6-P2 level in hepatocytes from fasted rats, or those pretreated with glucagon (<10-10M) but not those from fed rats. Glucose (>10mM) also increases the fructose-2,6-P2 and hexose-P levels in hepatocytes from starved rats, and its effect appears to be greater than that of insulin. Furthermore, insulin increases fructose-6-P,2-kinase activity and decreases cAMP levels in hepatocytes pretreated with glucagon, and it increases hexose-P concentration in hepatocytes from fasted rats. These results suggest that insulin antagonizes glucagon action by increasing fructose-6-P,2-kinase, decreasing cyclic AMP levels, and increasing hexose-P levels, resulting in increased concentrations of fructose-2,6-P2.
Original language | English (US) |
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Pages (from-to) | 394-401 |
Number of pages | 8 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 109 |
Issue number | 2 |
DOIs | |
State | Published - Nov 30 1982 |
Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology