The effect of estradiol on testicular testosterone biosynthesis

N. R. Kalla, B. C. Nisula, R. Menard, Donald (Lynn) Loriaux

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

The presence of an estrogen receptor in Leydig cell cytosol suggests that estrogen could have a direct action on Leydig cell function. We have studied the direct effects of estradiol (E2) on Leydig cell testosterone (T) biosynthesis using hypophysectomized rats treated daily for 4 days with 400 IU hCG (Pregnyl) and 1 IU human FSH (Pergonal), a model that eliminates the possibility of feedback effects of E2 gonadotropin secretion. E2 was administered in sc silastic capsules. Rats having capsules that contained E2 were compared to rats having capsules that were empty. E2 treatment increased plasma E2 concentration from 380 ± 56 to 670 ± 64 pg/ml (mean ± SE; P2 treatment could decrease plasma T were investigated, viz. changes in clearance of plasma T, changes in testicular hCG binding, and altered Leydig cell T biosynthesis. Plasma T clearance was not significantly changed by E2 treatment. hCG binding by testis homogenates was decreased from 4.70 ± 0.63 to 3.12 ± 0.26 ng hCG/testis (P2-treated animals. E2 treatment decreased testicular T content from 347 ± 70 to 226 ± 15 ng/testis (P2 in vivo can directly interfere with Leydig cell function. The principal manifestation of the inhibitory effect of E2 on T biosynthesis is an apparent reduction in 17-20 desmolase activity.

Original languageEnglish (US)
Pages (from-to)35-39
Number of pages5
JournalEndocrinology
Volume106
Issue number1
StatePublished - 1980
Externally publishedYes

Fingerprint

Leydig Cells
Testosterone
Estradiol
Capsules
Testis
Human Follicle Stimulating Hormone
Steroid 17-alpha-Hydroxylase
Menotropins
Chorionic Gonadotropin
Gonadotropins
Estrogen Receptors
Cytosol
Estrogens

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Kalla, N. R., Nisula, B. C., Menard, R., & Loriaux, D. L. (1980). The effect of estradiol on testicular testosterone biosynthesis. Endocrinology, 106(1), 35-39.

The effect of estradiol on testicular testosterone biosynthesis. / Kalla, N. R.; Nisula, B. C.; Menard, R.; Loriaux, Donald (Lynn).

In: Endocrinology, Vol. 106, No. 1, 1980, p. 35-39.

Research output: Contribution to journalArticle

Kalla, NR, Nisula, BC, Menard, R & Loriaux, DL 1980, 'The effect of estradiol on testicular testosterone biosynthesis', Endocrinology, vol. 106, no. 1, pp. 35-39.
Kalla, N. R. ; Nisula, B. C. ; Menard, R. ; Loriaux, Donald (Lynn). / The effect of estradiol on testicular testosterone biosynthesis. In: Endocrinology. 1980 ; Vol. 106, No. 1. pp. 35-39.
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