The effect of altered thyroid status on pituitary hormone messenger ribonucleic acid concentrations in the rat

Mary Samuels, M. E. Wierman, C. Wang, E. C. Ridgway

Research output: Contribution to journalArticle

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Abstract

To study the effects of altered thyroid status on pretranslational control of pituitary hormones, adult male rats were given propylthiouracil for 6 weeks and underwent the following studies. 1) Rats were injected with T3 at 10 μg/100 g BW daily for 10 days. 2) Rats were given T3 injections at 0, 0.01, 0.1, 1.0, or 10 μg/100 g BW for 10 days. 3) Rats were killed 0, 1, 6, or 24 h after a single injection of T3 at 10 μg/100 g BW or after 5 or 10 days of daily T3 injections. Pituitary mRNA concentrations of TSHβ, α-subunit, PRL, GH, POMC, FSHβ, and LHβ were determined for individual animals. Marked increases in TSHβ and α-subunit mRNAs occurred after PTU treatment, and these changes were reversed by 1.0 μg/100 g BW T3 and within 24 h of a single T3 injection of 10 μg/100 g BW. Further increases in the dose or time course of T3 administration led to a relatively greater suppression of TSHβ mRNA levels than α-subunit mRNA levels. In contrast, GH and PRL mRNA levels were low in hypothyroid animals, and both rose toward control levels with 0.1 μg/100 g BW T3 and by 24 h after a single T3 dose. Induction of hyperthyroidism did not further increase GH mRNA levels above control, but increased PRL mRNA levels 2-fold over control. No changes were seen in FSHβ, LHβ, or POMC mRNA levels with any treatment. Thus, studies of altered thyroid status in the rat reveal dose-response and time-course variability in the pretranslational control of TSHβ, α-subunit, GH, and PRL by thyroid hormone.

Original languageEnglish (US)
Pages (from-to)2277-2282
Number of pages6
JournalEndocrinology
Volume124
Issue number5
StatePublished - 1989
Externally publishedYes

Fingerprint

Pituitary Hormones
Thyroid Gland
RNA
Messenger RNA
Pro-Opiomelanocortin
Injections
Propylthiouracil
Hyperthyroidism
Thyroid Hormones
Reaction Time
Therapeutics

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

The effect of altered thyroid status on pituitary hormone messenger ribonucleic acid concentrations in the rat. / Samuels, Mary; Wierman, M. E.; Wang, C.; Ridgway, E. C.

In: Endocrinology, Vol. 124, No. 5, 1989, p. 2277-2282.

Research output: Contribution to journalArticle

Samuels, Mary ; Wierman, M. E. ; Wang, C. ; Ridgway, E. C. / The effect of altered thyroid status on pituitary hormone messenger ribonucleic acid concentrations in the rat. In: Endocrinology. 1989 ; Vol. 124, No. 5. pp. 2277-2282.
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