The E. coli gene encoding heat stable toxin is a bacterial transposon flanked by inverted repeats of IS1

Magdalene So, Fred Heffron, Brian J. McCarthy

    Research output: Contribution to journalArticle

    76 Scopus citations

    Abstract

    Restriction endonuclease subclones of the Escherichia coli gene encoding the heat stable (ST) toxin exhibit a stem and loop structure similar to those seen in many procaryotic transposons. An EcoRI DNA fragment encoding tetra-cycline (Tc) resistance but no transposition functions was spliced into the ST gene in one of these subclones. By monitoring Tcr, we were able to show that the ST gene transposes. Restriction and DNA sequence data strongly suggest that the ST transposon, Tn1681, is flanked by inverted repeats of IS1.

    Original languageEnglish (US)
    Pages (from-to)453-456
    Number of pages4
    JournalNature
    Volume277
    Issue number5696
    DOIs
    StatePublished - Dec 1 1979

    ASJC Scopus subject areas

    • General

    Fingerprint Dive into the research topics of 'The E. coli gene encoding heat stable toxin is a bacterial transposon flanked by inverted repeats of IS1'. Together they form a unique fingerprint.

  • Cite this