The E. coli gene encoding heat stable toxin is a bacterial transposon flanked by inverted repeats of IS1

Magdalene So; Fred Heffron; Brian J. McCarthy

doi:10.1038/277453a0

The E. coli gene encoding heat stable toxin is a bacterial transposon flanked by inverted repeats of IS1

Magdalene So, Fred Heffron, Brian J. McCarthy

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Abstract

Restriction endonuclease subclones of the Escherichia coli gene encoding the heat stable (ST) toxin exhibit a stem and loop structure similar to those seen in many procaryotic transposons. An EcoRI DNA fragment encoding tetra-cycline (Tc) resistance but no transposition functions was spliced into the ST gene in one of these subclones. By monitoring Tc^r, we were able to show that the ST gene transposes. Restriction and DNA sequence data strongly suggest that the ST transposon, Tn1681, is flanked by inverted repeats of IS1.

Original language	English (US)
Pages (from-to)	453-456
Number of pages	4
Journal	Nature
Volume	277
Issue number	5696
DOIs	https://doi.org/10.1038/277453a0
State	Published - 1979
Externally published	Yes

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abstract = "Restriction endonuclease subclones of the Escherichia coli gene encoding the heat stable (ST) toxin exhibit a stem and loop structure similar to those seen in many procaryotic transposons. An EcoRI DNA fragment encoding tetra-cycline (Tc) resistance but no transposition functions was spliced into the ST gene in one of these subclones. By monitoring Tcr, we were able to show that the ST gene transposes. Restriction and DNA sequence data strongly suggest that the ST transposon, Tn1681, is flanked by inverted repeats of IS1.",

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AU - So, Magdalene

AU - Heffron, Fred

AU - McCarthy, Brian J.

PY - 1979

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N2 - Restriction endonuclease subclones of the Escherichia coli gene encoding the heat stable (ST) toxin exhibit a stem and loop structure similar to those seen in many procaryotic transposons. An EcoRI DNA fragment encoding tetra-cycline (Tc) resistance but no transposition functions was spliced into the ST gene in one of these subclones. By monitoring Tcr, we were able to show that the ST gene transposes. Restriction and DNA sequence data strongly suggest that the ST transposon, Tn1681, is flanked by inverted repeats of IS1.

AB - Restriction endonuclease subclones of the Escherichia coli gene encoding the heat stable (ST) toxin exhibit a stem and loop structure similar to those seen in many procaryotic transposons. An EcoRI DNA fragment encoding tetra-cycline (Tc) resistance but no transposition functions was spliced into the ST gene in one of these subclones. By monitoring Tcr, we were able to show that the ST gene transposes. Restriction and DNA sequence data strongly suggest that the ST transposon, Tn1681, is flanked by inverted repeats of IS1.

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The E. coli gene encoding heat stable toxin is a bacterial transposon flanked by inverted repeats of IS1

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