The dynamics of mouse cytomegalovirus-specific CD4 T cell responses during acute and latent infection

Senta M. Walton, Philippe Wyrsch, Michael W. Munks, Albert Zimmermann, Hartmut Hengel, Ann B. Hill, Annette Oxenius

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

The dynamics of mouse cytomegalovirus (MCMV)-specific CD4 T cell responses and the mechanisms by which these cells contribute to viral control are not well understood, mainly due to lack of appropriate tools to characterize MCMV-specific CD4 T cells. We therefore generated MCMV-specific CD4 T cell hybridomas, then used an MCMV expression library and overlapping peptides to identify CD4 T cell epitopes. We used these novel tools to study the long-term kinetics and organ distribution of MCMV-specific CD4 T cells in comparison to MCMV-specific CD8 T cell responses. We demonstrate that the overall MCMVspecific CD4 T cell response stabilizes during the latent stage, which stands in contrast to subpopulations of MCMV-specific CD8 T cells and HCMV-specific CD4 T cells which accumulate over the course of CMV latency. Furthermore, MCMV-specific CD4 T cells displayed a Th1 phenotype, secreting high levels of IFN-γand TNF-αand to some extent IL-2, cytokines which are involved in protection from CMV disease.

Original languageEnglish (US)
Pages (from-to)1128-1134
Number of pages7
JournalJournal of Immunology
Volume181
Issue number2
DOIs
StatePublished - Jul 15 2008

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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