The drosophila cell survival gene discs lost encodes a cytoplasmic codanin-1-like protein, not a homolog of tight junction PDZ protein Patj

Jan Pielage, Tobias Stork, Ingrid Bunse, Christian Klämbt

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

The Drosophila gene discs lost (dlt) has been reported to encode a homolog of the vertebrate tight junction PDZ protein Patj, and was thought to play a role in cell polarity. Using rescue experiments and sequence analyses, we show that dlt mutations disrupt the Drosophila Codanin-1 homolog, a cytoplasmic protein, and not the PDZ protein. Mutations in human Codanin-1 are associated with congenital dyserythropoietic anemia type I (CDA I). In Drosophila, the genomic organization of dlt is unusual. dlt shares its first untranslated exon with α-spectrin, and both genes are coexpressed throughout development. We show that dlt is not required for cell polarity but is needed for cell survival and cell cycle progression. Finally, we present evidence that the PDZ protein previously thought to be encoded by dlt is not required for viability. We propose to rename this PDZ protein after its vertebrate homolog, Patj (Pals-associated tight junction protein).

Original languageEnglish (US)
Pages (from-to)841-851
Number of pages11
JournalDevelopmental Cell
Volume5
Issue number6
DOIs
StatePublished - Dec 1 2003

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Developmental Biology
  • Cell Biology

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