The DNA-binding subunit of human transcription factor IID can interact with the TATA box as a multimer

Ray Jupp, Osvaldo Flores, Jay Nelson, Peter Ghazal

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Transcription initiation from eukaryotic protein-coding genes is a complex process that minimally requires RNA polymerase (pol) II (B) and at least seven general transcription factors. The 38-kDa subunit (TBP) of the human general transcription factor TFIID recognizes the TATA sequence element and initiates the assembly of the other general transcription factors and RNA pol II. It is believed, based on experiments with yeast recombinant protein, that TBP binds as a monomer to DNA. Using purified recombinant human TBP protein we find that TBP interacts with the TATA element as both a monomer and a dimer. The multimeric binding of TBP to DNA revealed by this study has important implications for the role of TBP in transcription initiation and suggests novel mechanisms whereby other transcription factors may interact with a RNA pol II preinitiation complex.

Original languageEnglish (US)
Pages (from-to)16105-16108
Number of pages4
JournalJournal of Biological Chemistry
Volume268
Issue number22
StatePublished - Aug 5 1993

Fingerprint

Transcription Factor TFIID
TATA Box
RNA Polymerase II
General Transcription Factors
DNA
Transcription
Monomers
Fungal Proteins
Recombinant Proteins
Dimers
Yeast
Transcription Factors
Genes
tributyl phosphate
Proteins
Experiments

ASJC Scopus subject areas

  • Biochemistry

Cite this

The DNA-binding subunit of human transcription factor IID can interact with the TATA box as a multimer. / Jupp, Ray; Flores, Osvaldo; Nelson, Jay; Ghazal, Peter.

In: Journal of Biological Chemistry, Vol. 268, No. 22, 05.08.1993, p. 16105-16108.

Research output: Contribution to journalArticle

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