The distribution of nuclear DNA from human brain-tumor cells. Flow cytometric studies

T. Hoshino, K. Nomura, C. B. Wilson, K. D. Knebel, Joe Gray

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Flow cytometry (FCM) is a technique that measures the quantity of DNA contained in individual nuclei and records a frequency distribution of the DNA content per nucleus in the sampled cell population. Nuclei from a variety of human brain tumor types were isolated by means of tissue grinding, purified by centrifugation through 40% sucrose (15 min at 4,000 rpm), fixed with 10% formalin, stained with acriflavin-Feulgen, and analyzed by FCM. Profiles of DNA distribution in histologically benign tumors, such as meningiomas, pituitary adenomas, neuroblastomas, and low-grade astrocytomas, revealed a large diploid population (2C) with a few nuclei in DNA synthesis, as well as a small premitotic population (G 2 cells) that contains a 4C DNA complement. In contrast, malignant gliomas, including glioblastomas, consist of more cells in DNA synthesis; these tumor cells show a highly variable distribution of ploidy consisting not only of diploid, and/or aneuploid, but also of triploid, tetraploid, and possibly octaploid populations. Also, a large variability between different regions of each tumor was always observed. In contrast, metastatic brain tumors, despite the fact that they contain a considerable number of cells undergoing DNA synthesis, demonstrate little variability within each individual tumor. The ability to rapidly characterize the cell populations of human brain tumors with FCM may enhance the effectiveness of their clinical management.

Original languageEnglish (US)
Pages (from-to)13-21
Number of pages9
JournalJournal of Neurosurgery
Volume49
Issue number1
StatePublished - 1978
Externally publishedYes

Fingerprint

Brain Neoplasms
DNA
Flow Cytometry
Population
Diploidy
Neoplasms
Triploidy
Gastrin-Secreting Cells
Tetraploidy
Ploidies
Astrocytoma
Pituitary Neoplasms
Meningioma
Aneuploidy
Glioblastoma
Neuroblastoma
Centrifugation
Glioma
Formaldehyde
Sucrose

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Hoshino, T., Nomura, K., Wilson, C. B., Knebel, K. D., & Gray, J. (1978). The distribution of nuclear DNA from human brain-tumor cells. Flow cytometric studies. Journal of Neurosurgery, 49(1), 13-21.

The distribution of nuclear DNA from human brain-tumor cells. Flow cytometric studies. / Hoshino, T.; Nomura, K.; Wilson, C. B.; Knebel, K. D.; Gray, Joe.

In: Journal of Neurosurgery, Vol. 49, No. 1, 1978, p. 13-21.

Research output: Contribution to journalArticle

Hoshino, T, Nomura, K, Wilson, CB, Knebel, KD & Gray, J 1978, 'The distribution of nuclear DNA from human brain-tumor cells. Flow cytometric studies', Journal of Neurosurgery, vol. 49, no. 1, pp. 13-21.
Hoshino, T. ; Nomura, K. ; Wilson, C. B. ; Knebel, K. D. ; Gray, Joe. / The distribution of nuclear DNA from human brain-tumor cells. Flow cytometric studies. In: Journal of Neurosurgery. 1978 ; Vol. 49, No. 1. pp. 13-21.
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