The distribution of neuronal calcium sensor-I protein in the developing and adult rat retina

Anna J. Reynolds, Selena E. Bartlett, Catherine Morgans

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We have examined the distribution of the neuronal calcium-binding protein, neuronal calcium sensor I (NCS-1) in the developing and adult rat retina using subcellular fractionation of the rat retina and immunohistochemistry. NCS-1 immunoreactivity was situated primarily in the ganglion cells, a class of amacrine cells, and in the inner plexiform layer (IPL). During development, NCS-1 protein expression closely followed that of the synaptic vesicle protein, synaptophysin, increasing dramatically in the IPL at postnatal day 3, the time when conventional synapses are formed in the retina. These findings suggest that NCS-1 plays a role in synaptogenesis in the retina and in synaptic transmission at conventional synapses but not ribbon synapses in the adult rat retina.

Original languageEnglish (US)
Pages (from-to)725-728
Number of pages4
JournalNeuroReport
Volume12
Issue number4
StatePublished - Mar 26 2001
Externally publishedYes

Fingerprint

Neuronal Calcium-Sensor Proteins
Retina
Synapses
Amacrine Cells
Synaptophysin
Calcium-Binding Proteins
Synaptic Vesicles
Synaptic Transmission
Ganglia
Proteins
Immunohistochemistry
Calcium

Keywords

  • Frequenin
  • NCS-1
  • Rat
  • Retina
  • Synaptogenesis

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The distribution of neuronal calcium sensor-I protein in the developing and adult rat retina. / Reynolds, Anna J.; Bartlett, Selena E.; Morgans, Catherine.

In: NeuroReport, Vol. 12, No. 4, 26.03.2001, p. 725-728.

Research output: Contribution to journalArticle

Reynolds, Anna J. ; Bartlett, Selena E. ; Morgans, Catherine. / The distribution of neuronal calcium sensor-I protein in the developing and adult rat retina. In: NeuroReport. 2001 ; Vol. 12, No. 4. pp. 725-728.
@article{9d7f7d77b42644ceb523f0c54f2c45c8,
title = "The distribution of neuronal calcium sensor-I protein in the developing and adult rat retina",
abstract = "We have examined the distribution of the neuronal calcium-binding protein, neuronal calcium sensor I (NCS-1) in the developing and adult rat retina using subcellular fractionation of the rat retina and immunohistochemistry. NCS-1 immunoreactivity was situated primarily in the ganglion cells, a class of amacrine cells, and in the inner plexiform layer (IPL). During development, NCS-1 protein expression closely followed that of the synaptic vesicle protein, synaptophysin, increasing dramatically in the IPL at postnatal day 3, the time when conventional synapses are formed in the retina. These findings suggest that NCS-1 plays a role in synaptogenesis in the retina and in synaptic transmission at conventional synapses but not ribbon synapses in the adult rat retina.",
keywords = "Frequenin, NCS-1, Rat, Retina, Synaptogenesis",
author = "Reynolds, {Anna J.} and Bartlett, {Selena E.} and Catherine Morgans",
year = "2001",
month = "3",
day = "26",
language = "English (US)",
volume = "12",
pages = "725--728",
journal = "NeuroReport",
issn = "0959-4965",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - The distribution of neuronal calcium sensor-I protein in the developing and adult rat retina

AU - Reynolds, Anna J.

AU - Bartlett, Selena E.

AU - Morgans, Catherine

PY - 2001/3/26

Y1 - 2001/3/26

N2 - We have examined the distribution of the neuronal calcium-binding protein, neuronal calcium sensor I (NCS-1) in the developing and adult rat retina using subcellular fractionation of the rat retina and immunohistochemistry. NCS-1 immunoreactivity was situated primarily in the ganglion cells, a class of amacrine cells, and in the inner plexiform layer (IPL). During development, NCS-1 protein expression closely followed that of the synaptic vesicle protein, synaptophysin, increasing dramatically in the IPL at postnatal day 3, the time when conventional synapses are formed in the retina. These findings suggest that NCS-1 plays a role in synaptogenesis in the retina and in synaptic transmission at conventional synapses but not ribbon synapses in the adult rat retina.

AB - We have examined the distribution of the neuronal calcium-binding protein, neuronal calcium sensor I (NCS-1) in the developing and adult rat retina using subcellular fractionation of the rat retina and immunohistochemistry. NCS-1 immunoreactivity was situated primarily in the ganglion cells, a class of amacrine cells, and in the inner plexiform layer (IPL). During development, NCS-1 protein expression closely followed that of the synaptic vesicle protein, synaptophysin, increasing dramatically in the IPL at postnatal day 3, the time when conventional synapses are formed in the retina. These findings suggest that NCS-1 plays a role in synaptogenesis in the retina and in synaptic transmission at conventional synapses but not ribbon synapses in the adult rat retina.

KW - Frequenin

KW - NCS-1

KW - Rat

KW - Retina

KW - Synaptogenesis

UR - http://www.scopus.com/inward/record.url?scp=0035953138&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035953138&partnerID=8YFLogxK

M3 - Article

C2 - 11277572

AN - SCOPUS:0035953138

VL - 12

SP - 725

EP - 728

JO - NeuroReport

JF - NeuroReport

SN - 0959-4965

IS - 4

ER -