THE DISTANCE BETWEEN THE PHYTOCHROME CHROMOPHORE AND THE N‐TERMINAL CHAIN DECREASES DURING PHOTOTRANSFORMATION. A NOVEL FLUORESCENCE ENERGY TRANSFER METHOD USING LABELED ANTIBODY FRAGMENTS

David L. Farrens; Marie‐Michèle ‐M Cordonnier; Lee H. Pratt; Pill‐Soon ‐S Song

doi:10.1111/j.1751-1097.1992.tb02227.x

THE DISTANCE BETWEEN THE PHYTOCHROME CHROMOPHORE AND THE N‐TERMINAL CHAIN DECREASES DURING PHOTOTRANSFORMATION. A NOVEL FLUORESCENCE ENERGY TRANSFER METHOD USING LABELED ANTIBODY FRAGMENTS

David L. Farrens, Marie‐Michèle ‐M Cordonnier, Lee H. Pratt, Pill‐Soon ‐S Song

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

A novel antibody‐fluorescence method has been developed to elucidate the chromophore topography in phytochrome as it undergoes a photochromic transformation. Förster energy transfer from N‐terminal bound, fluorescently labeled Oat‐25 Fab antibody fragments to the phytochrome chromophore was measured. The results suggest that the chromophore moves relative to the N‐terminus upon the Pr → Pfr phototransformation. This conclusion is consistent with previous models which have proposed a reorientation and an interaction of the Pfr chromophore with the N‐terminus. The method described appears to be the first study of a Forster energy transfer measurement using a donor‐label attached to a Fab fragment of a photosensor protein.

Original language	English (US)
Pages (from-to)	725-733
Number of pages	9
Journal	Photochemistry and photobiology
Volume	56
Issue number	5
DOIs	https://doi.org/10.1111/j.1751-1097.1992.tb02227.x
State	Published - Nov 1992
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry

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10.1111/j.1751-1097.1992.tb02227.x

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THE DISTANCE BETWEEN THE PHYTOCHROME CHROMOPHORE AND THE N‐TERMINAL CHAIN DECREASES DURING PHOTOTRANSFORMATION. A NOVEL FLUORESCENCE ENERGY TRANSFER METHOD USING LABELED ANTIBODY FRAGMENTS. / Farrens, David L.; Cordonnier, Marie‐Michèle ‐M; Pratt, Lee H. et al.
In: Photochemistry and photobiology, Vol. 56, No. 5, 11.1992, p. 725-733.

Research output: Contribution to journal › Article › peer-review

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title = "THE DISTANCE BETWEEN THE PHYTOCHROME CHROMOPHORE AND THE N‐TERMINAL CHAIN DECREASES DURING PHOTOTRANSFORMATION. A NOVEL FLUORESCENCE ENERGY TRANSFER METHOD USING LABELED ANTIBODY FRAGMENTS",

abstract = "A novel antibody‐fluorescence method has been developed to elucidate the chromophore topography in phytochrome as it undergoes a photochromic transformation. F{\"o}rster energy transfer from N‐terminal bound, fluorescently labeled Oat‐25 Fab antibody fragments to the phytochrome chromophore was measured. The results suggest that the chromophore moves relative to the N‐terminus upon the Pr → Pfr phototransformation. This conclusion is consistent with previous models which have proposed a reorientation and an interaction of the Pfr chromophore with the N‐terminus. The method described appears to be the first study of a Forster energy transfer measurement using a donor‐label attached to a Fab fragment of a photosensor protein.",

author = "Farrens, {David L.} and Cordonnier, {Marie‐Mich{\`e}le ‐M} and Pratt, {Lee H.} and Song, {Pill‐Soon ‐S}",

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AU - Farrens, David L.

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AB - A novel antibody‐fluorescence method has been developed to elucidate the chromophore topography in phytochrome as it undergoes a photochromic transformation. Förster energy transfer from N‐terminal bound, fluorescently labeled Oat‐25 Fab antibody fragments to the phytochrome chromophore was measured. The results suggest that the chromophore moves relative to the N‐terminus upon the Pr → Pfr phototransformation. This conclusion is consistent with previous models which have proposed a reorientation and an interaction of the Pfr chromophore with the N‐terminus. The method described appears to be the first study of a Forster energy transfer measurement using a donor‐label attached to a Fab fragment of a photosensor protein.

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THE DISTANCE BETWEEN THE PHYTOCHROME CHROMOPHORE AND THE N‐TERMINAL CHAIN DECREASES DURING PHOTOTRANSFORMATION. A NOVEL FLUORESCENCE ENERGY TRANSFER METHOD USING LABELED ANTIBODY FRAGMENTS

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