The direct cooling of the preoptic-hypothalamic area elicits the release of thyroid stimulating hormone during wakefulness but not during REM sleep

Davide Martelli, Marco Luppi, Matteo Cerri, Domenico Tupone, Marco Mastrotto, Emanuele Perez, Giovanni Zamboni, Roberto Amici

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Thermoregulatory responses to temperature changes are not operant during REM sleep (REMS), but fully operant in non-REM sleep and wakefulness. The specificity of the relationship between REMS and the impairment of thermoregulation was tested by eliciting the reflex release of Thyrotropin Releasing Hormone (TRH), which is integrated at hypothalamic level. By inducing the sequential secretion of Thyroid Stimulating Hormone (TSH) and Thyroid Hormone, TRH intervenes in the regulation of obligatory and non-shivering thermogenesis. Experiments were performed on male albino rats implanted with epidural electrodes for EEG recording and 2 silver-copper wire thermodes, bilaterally placed in the preoptic-hypothalamic area (POA) and connected to small thermoelectric heat pumps driven by a low-voltage high current DC power supply. In preliminary experiments, a thermistor was added in order to measure hypothalamic temperature. The activation of TRH hypophysiotropic neurons by the thermode cooling of POA was indirectly assessed, in conditions in which thermoregulation was either fully operant (wakefulness) or not operant (REMS), by a radioimmunoassay determination of plasmatic levels of TSH. Different POA cooling were performed for 120 s or 40 s at current intensities of 80 mA and 125 mA, respectively. At both current intensities, POA cooling elicited, with respect to control values (no cooling current), a significant increase in plasmatic TSH levels in wakefulness, but not during REMS. These results confirm the inactivation of POA thermal sensitivity during REMS and show, for the first time, that this inactivation concerns also the fundamental endocrine control of non-shivering thermogenesis.

Original languageEnglish (US)
Article numbere87793
JournalPLoS One
Volume9
Issue number2
DOIs
StatePublished - Feb 3 2014

Fingerprint

Preoptic Area
thyrotropin
Wakefulness
REM Sleep
Thyrotropin
sleep
cooling
Thyrotropin-Releasing Hormone
Cooling
thyrotropin-releasing hormone
Thermogenesis
Body Temperature Regulation
thermoregulation
heat production
Hot Temperature
inactivation
Electric Power Supplies
Temperature
heat pumps
Thermistors

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

The direct cooling of the preoptic-hypothalamic area elicits the release of thyroid stimulating hormone during wakefulness but not during REM sleep. / Martelli, Davide; Luppi, Marco; Cerri, Matteo; Tupone, Domenico; Mastrotto, Marco; Perez, Emanuele; Zamboni, Giovanni; Amici, Roberto.

In: PLoS One, Vol. 9, No. 2, e87793, 03.02.2014.

Research output: Contribution to journalArticle

Martelli, Davide ; Luppi, Marco ; Cerri, Matteo ; Tupone, Domenico ; Mastrotto, Marco ; Perez, Emanuele ; Zamboni, Giovanni ; Amici, Roberto. / The direct cooling of the preoptic-hypothalamic area elicits the release of thyroid stimulating hormone during wakefulness but not during REM sleep. In: PLoS One. 2014 ; Vol. 9, No. 2.
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