The degradation of mitomycin C under various storage methods

Robert M. Kinast, Kiran K. Akula, Andrea De Barber, Gordon T. Barker, Stuart K. Gardiner, Emily Whitson, Steve L. Mansberger

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Purpose: To compare the effects of common pharmacy preparation and storage conditions on the stability of mitomycin C (MMC) in solution. Methods: We used C18 reversed-phase high-performance liquid chromatography to determine the stability of 0.4 mg/mL MMC solutions, and liquid chromatography-electrospray ionization-mass spectrometry to identify degradation products. Conditions compared were: compounding and storage by refrigeration (1 and 2 wk), freezing (23 d), shipment "on-ice" (1 mo frozen followed by 1-wk refrigeration), and immediately compounding dry powder (Mitosol; Mobius Therapeutics LLC). We tested 3 samples for each storage method when samples reached room temperature (time 0), and then 1, 4, and 24 hours later. We used MMC peak area as a percentage of total (MMC plus degradants) area detected with high-performance liquid chromatography as a measure of stability. Results: We assessed MMC stability for 5 preparation and storage methods at 4 timepoints (with n=3 per timepoint). At time 0, we found similar stabilities for MMC (F=0.72, P=0.599) between all 5 storage methods: 1-week refrigerated (97.9±0.2%), dry powder (97.5±0.3%), 2-week refrigerated (96.9±0.2%), 23-day frozen (96.7±3.1%), and shipment on-ice (96.0±1.2%). However, MMC demonstrated significant degradation over a 24-hour period with 2-week refrigeration (95.7±0.3%, β=-0.1%/h, P

Original languageEnglish (US)
Pages (from-to)477-481
Number of pages5
JournalJournal of Glaucoma
Volume25
Issue number6
DOIs
StatePublished - 2016

Keywords

  • degradation
  • high-performance liquid chromatography
  • liquid chromatography-mass spectrometry
  • mitomycin C
  • stability

ASJC Scopus subject areas

  • Ophthalmology

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