The degradation of mitomycin C under various storage methods

Robert M. Kinast, Kiran K. Akula, Andrea De Barber, Gordon T. Barker, Stuart K. Gardiner, Emily Whitson, Steve L. Mansberger

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Purpose: To compare the effects of common pharmacy preparation and storage conditions on the stability of mitomycin C (MMC) in solution. Methods: We used C18 reversed-phase high-performance liquid chromatography to determine the stability of 0.4 mg/mL MMC solutions, and liquid chromatography-electrospray ionization-mass spectrometry to identify degradation products. Conditions compared were: compounding and storage by refrigeration (1 and 2 wk), freezing (23 d), shipment "on-ice" (1 mo frozen followed by 1-wk refrigeration), and immediately compounding dry powder (Mitosol; Mobius Therapeutics LLC). We tested 3 samples for each storage method when samples reached room temperature (time 0), and then 1, 4, and 24 hours later. We used MMC peak area as a percentage of total (MMC plus degradants) area detected with high-performance liquid chromatography as a measure of stability. Results: We assessed MMC stability for 5 preparation and storage methods at 4 timepoints (with n=3 per timepoint). At time 0, we found similar stabilities for MMC (F=0.72, P=0.599) between all 5 storage methods: 1-week refrigerated (97.9±0.2%), dry powder (97.5±0.3%), 2-week refrigerated (96.9±0.2%), 23-day frozen (96.7±3.1%), and shipment on-ice (96.0±1.2%). However, MMC demonstrated significant degradation over a 24-hour period with 2-week refrigeration (95.7±0.3%, β=-0.1%/h, P

Original languageEnglish (US)
Pages (from-to)477-481
Number of pages5
JournalJournal of Glaucoma
Volume25
Issue number6
DOIs
StatePublished - 2016

Fingerprint

Mitomycin
Refrigeration
Ice
Powders
High Pressure Liquid Chromatography
Electrospray Ionization Mass Spectrometry
Reverse-Phase Chromatography
Liquid Chromatography
Freezing
Temperature

Keywords

  • degradation
  • high-performance liquid chromatography
  • liquid chromatography-mass spectrometry
  • mitomycin C
  • stability

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Kinast, R. M., Akula, K. K., De Barber, A., Barker, G. T., Gardiner, S. K., Whitson, E., & Mansberger, S. L. (2016). The degradation of mitomycin C under various storage methods. Journal of Glaucoma, 25(6), 477-481. https://doi.org/10.1097/IJG.0000000000000287

The degradation of mitomycin C under various storage methods. / Kinast, Robert M.; Akula, Kiran K.; De Barber, Andrea; Barker, Gordon T.; Gardiner, Stuart K.; Whitson, Emily; Mansberger, Steve L.

In: Journal of Glaucoma, Vol. 25, No. 6, 2016, p. 477-481.

Research output: Contribution to journalArticle

Kinast, RM, Akula, KK, De Barber, A, Barker, GT, Gardiner, SK, Whitson, E & Mansberger, SL 2016, 'The degradation of mitomycin C under various storage methods', Journal of Glaucoma, vol. 25, no. 6, pp. 477-481. https://doi.org/10.1097/IJG.0000000000000287
Kinast RM, Akula KK, De Barber A, Barker GT, Gardiner SK, Whitson E et al. The degradation of mitomycin C under various storage methods. Journal of Glaucoma. 2016;25(6):477-481. https://doi.org/10.1097/IJG.0000000000000287
Kinast, Robert M. ; Akula, Kiran K. ; De Barber, Andrea ; Barker, Gordon T. ; Gardiner, Stuart K. ; Whitson, Emily ; Mansberger, Steve L. / The degradation of mitomycin C under various storage methods. In: Journal of Glaucoma. 2016 ; Vol. 25, No. 6. pp. 477-481.
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