The Cu(A) domain of Thermus thermophilus ba3-type cytochrome C oxidase at 1.6 Å resolution

P. A. Williams, Ninian Blackburn, D. Sanders, H. Bellamy, E. A. Stura, J. A. Fee, D. E. McRee

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

The structure of the Cu(A)-containing, extracellular domain of Thermus thermophilus ba3-type cytochrome c oxidase has been determined to 1.6 Å resolution using multiple X-ray wavelength anomalous dispersion (MAD). The Cu2S2 cluster forms a planar rhombus with a copper-copper distance of 2.51 ± 0.03 Å. X-ray absorption fine-structure (EXAFS) studies show that this distance is unchanged by crystallization. The Cu(A) center is asymmetrical; one copper is tetrahedrally coordinated to two bridging cysteine thiolates, one histidine nitrogen and one methionine sulfur, while the other is trigonally coordinated by the two cysteine thiolates and a histidine nitrogen. Combined sequence-structure alignment of amino acid sequences reveals conserved interactions between cytochrome c oxidase subunits I and II.

Original languageEnglish (US)
Pages (from-to)509-516
Number of pages8
JournalNature Structural Biology
Volume6
Issue number6
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Thermus thermophilus
Electron Transport Complex IV
Copper
Oxidoreductases
Histidine
Nitrogen
X-Rays
Sequence Alignment
X ray absorption
Crystallization
Sulfur
Methionine
Amino Acid Sequence
Amino Acids
X rays
Wavelength
cytochrome ba3
cysteine thiolate

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology

Cite this

Williams, P. A., Blackburn, N., Sanders, D., Bellamy, H., Stura, E. A., Fee, J. A., & McRee, D. E. (1999). The Cu(A) domain of Thermus thermophilus ba3-type cytochrome C oxidase at 1.6 Å resolution. Nature Structural Biology, 6(6), 509-516. https://doi.org/10.1038/9274

The Cu(A) domain of Thermus thermophilus ba3-type cytochrome C oxidase at 1.6 Å resolution. / Williams, P. A.; Blackburn, Ninian; Sanders, D.; Bellamy, H.; Stura, E. A.; Fee, J. A.; McRee, D. E.

In: Nature Structural Biology, Vol. 6, No. 6, 1999, p. 509-516.

Research output: Contribution to journalArticle

Williams, PA, Blackburn, N, Sanders, D, Bellamy, H, Stura, EA, Fee, JA & McRee, DE 1999, 'The Cu(A) domain of Thermus thermophilus ba3-type cytochrome C oxidase at 1.6 Å resolution', Nature Structural Biology, vol. 6, no. 6, pp. 509-516. https://doi.org/10.1038/9274
Williams, P. A. ; Blackburn, Ninian ; Sanders, D. ; Bellamy, H. ; Stura, E. A. ; Fee, J. A. ; McRee, D. E. / The Cu(A) domain of Thermus thermophilus ba3-type cytochrome C oxidase at 1.6 Å resolution. In: Nature Structural Biology. 1999 ; Vol. 6, No. 6. pp. 509-516.
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