The CRE-binding protein dCREB-A is required for Drosophila embryonic development

Ronald E. Rose, Nicole M. Gallaher, Deborah J. Andrew, Richard Goodman, Sarah M. Smolik

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We have previously described the cloning of a cyclic AMP response- element (CRE)-binding protein, dCREB-A, in Drosophila melanogaster that is similar to the mammalian CRE-binding protein CREB, dCREB-A is a member of the bZIP family of transcription factors, shows specific binding to the (CRE), and can activate transcription in cell culture. In this report, we describe the gene structure for dCREB-A, protein expression patterns throughout development and the necessary role for this gene in embryogenesis. The 4.5- kb transcript is encoded in six exons that are distributed over 21 kb of DNA. There are seven start sites and no TATA consensus sequences upstream. The dCREB-A protein is expressed in the nuclei of the embryonic salivary gland, proventriculus and stomadeum. Late in embryogenesis, tracheal cell nuclei and specific nuclei within the segments show staining with anti-dCREB-A antibodies. In adult female ovaries, dCREB-A is expressed in the stage 9 through stage 11 follicle cell nuclei. Null mutations of the dCREB-A gene give rise to animals that no longer express dCREB-A protein and die late in embryogenesis before or at hatching. The absolute requirement of dCREB-A for embryogenesis demonstrates a nonredundant function for a CRE-binding protein that will be useful in studying the role of specific signal transduction cascades in development.

Original languageEnglish (US)
Pages (from-to)595-606
Number of pages12
JournalGenetics
Volume146
Issue number2
StatePublished - Jun 1997

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Cyclic AMP Response Element-Binding Protein
Drosophila
Embryonic Development
Cell Nucleus
Proventriculus
Basic-Leucine Zipper Transcription Factors
Genes
Proteins
Consensus Sequence
Response Elements
Salivary Glands
Drosophila melanogaster
Cyclic AMP
Organism Cloning
Ovary
Exons
Signal Transduction
Cell Culture Techniques
Staining and Labeling
Mutation

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Rose, R. E., Gallaher, N. M., Andrew, D. J., Goodman, R., & Smolik, S. M. (1997). The CRE-binding protein dCREB-A is required for Drosophila embryonic development. Genetics, 146(2), 595-606.

The CRE-binding protein dCREB-A is required for Drosophila embryonic development. / Rose, Ronald E.; Gallaher, Nicole M.; Andrew, Deborah J.; Goodman, Richard; Smolik, Sarah M.

In: Genetics, Vol. 146, No. 2, 06.1997, p. 595-606.

Research output: Contribution to journalArticle

Rose, RE, Gallaher, NM, Andrew, DJ, Goodman, R & Smolik, SM 1997, 'The CRE-binding protein dCREB-A is required for Drosophila embryonic development', Genetics, vol. 146, no. 2, pp. 595-606.
Rose RE, Gallaher NM, Andrew DJ, Goodman R, Smolik SM. The CRE-binding protein dCREB-A is required for Drosophila embryonic development. Genetics. 1997 Jun;146(2):595-606.
Rose, Ronald E. ; Gallaher, Nicole M. ; Andrew, Deborah J. ; Goodman, Richard ; Smolik, Sarah M. / The CRE-binding protein dCREB-A is required for Drosophila embryonic development. In: Genetics. 1997 ; Vol. 146, No. 2. pp. 595-606.
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