The cochlear pericytes

Xiao Shi, Weijiu Han, Hiroshi Yamamoto, Wenxue Tang, Xi Lin, Ruijuan Xiu, Dennis Trune, Alfred Nuttall

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Objectives: Cochlear pericytes are not well characterized. The aim of this study was to further advance the characterization of cochlear pericyte location and distribution, with particular focus on pericyte-related proteins on the capillaries of the cochlear lateral wall that are functionally integral to structure, contraction, and gap junction transport. Materials and Methods: Cochlear pericytes were identified by the immunofluorescence labeling of pericyte marker proteins, including alpha-smooth muscle actin (α-SMA), desmin, Thy-1, tropomyosin, and NG2, and by morphological identification, using fluorescence, electron, and differential interference contrast microscopy. Results: Pericytes were predominately found in the capillary network of the cochlear lateral wall, with considerable morphological heterogeneity across different types of microvessels. For example, pericytes on the vessels of the spiral ligament (V/SL) strongly expressed a gap junction protein, connexin 40, and were positive for α-SMA, tropomyosin, and desmin. In contrast, pericytes on the vessels of the stria vascularis (V/SV) were positive for desmin, and were negative for α-SMA and tropomyosin. Conclusions: The capillary networks of the cochlear lateral wall comprise a rich population of pericytes. These pericytes are morphologically heterogeneous, with protein expression potentially indicative of function.

Original languageEnglish (US)
Pages (from-to)515-529
Number of pages15
JournalMicrocirculation
Volume15
Issue number6
DOIs
StatePublished - Aug 2008

Fingerprint

Pericytes
Cochlea
Tropomyosin
Desmin
Spiral Ligament of Cochlea
Interference Microscopy
Stria Vascularis
Proteins
Connexins
Gap Junctions
Microvessels
Fluorescent Antibody Technique
Smooth Muscle
Actins
Fluorescence
Electrons

Keywords

  • α-SMA
  • Cochlear pericyte
  • Connexin 40
  • Desmin
  • NG2
  • Tropomyosin

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Cardiology and Cardiovascular Medicine

Cite this

Shi, X., Han, W., Yamamoto, H., Tang, W., Lin, X., Xiu, R., ... Nuttall, A. (2008). The cochlear pericytes. Microcirculation, 15(6), 515-529. https://doi.org/10.1080/10739680802047445

The cochlear pericytes. / Shi, Xiao; Han, Weijiu; Yamamoto, Hiroshi; Tang, Wenxue; Lin, Xi; Xiu, Ruijuan; Trune, Dennis; Nuttall, Alfred.

In: Microcirculation, Vol. 15, No. 6, 08.2008, p. 515-529.

Research output: Contribution to journalArticle

Shi, X, Han, W, Yamamoto, H, Tang, W, Lin, X, Xiu, R, Trune, D & Nuttall, A 2008, 'The cochlear pericytes', Microcirculation, vol. 15, no. 6, pp. 515-529. https://doi.org/10.1080/10739680802047445
Shi X, Han W, Yamamoto H, Tang W, Lin X, Xiu R et al. The cochlear pericytes. Microcirculation. 2008 Aug;15(6):515-529. https://doi.org/10.1080/10739680802047445
Shi, Xiao ; Han, Weijiu ; Yamamoto, Hiroshi ; Tang, Wenxue ; Lin, Xi ; Xiu, Ruijuan ; Trune, Dennis ; Nuttall, Alfred. / The cochlear pericytes. In: Microcirculation. 2008 ; Vol. 15, No. 6. pp. 515-529.
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