The coactivator CBP stimulates human T-cell lymphotrophic virus type I tax transactivation in vitro

Fatah Kashanchi, Janet F. Duvall, Roland P S Kwok, James Lundblad, Richard Goodman, John N. Brady

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Tax interacts with the cellular cyclic AMP-responsive element binding protein (CREB) and facilitates the binding of the coactivator CREB binding protein (CBP), forming a multimeric complex on the cyclic AMP-responsive element (CRE)-like sites in the human T-cell lymphotrophic virus type I (HTLV-I) promoter. The trimeric complex is believed to recruit additional regulatory proteins to the HTLV-I long terminal repeat, but there has been no direct evidence that CBP is required for Tax-mediated transactivation. We present evidence that Tax and CBP activate transcription from the HTLV-I 21 base pair repeats on naked DNA templates. Transcriptional activation of the HTLV-I sequences required both Tax and CBP and could be mediated by either the N-terminal activation domain of CBP or the full-length protein. Fluorescence polarization binding assays indicated that CBP does not markedly enhance the affinity of Tax for the trimeric complex. Transcription analyses suggest that CBP activates Tax-dependent transcription by promoting transcriptional initiation and reinitiation. The ability of CBP to activate the HTLV-I promoter does not involve the stabilization of Tax binding, but rather depends upon gene activation properties of the co-activator that function in the context of a naked DNA template.

Original languageEnglish (US)
Pages (from-to)34646-34652
Number of pages7
JournalJournal of Biological Chemistry
Volume273
Issue number51
DOIs
StatePublished - Dec 18 1998

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CREB-Binding Protein
T-cells
Taxation
Viruses
Transcriptional Activation
T-Lymphocytes
Transcription
Cyclic AMP
Carrier Proteins
Chemical activation
Protein Binding
Activator Appliances
Fluorescence Polarization
Terminal Repeat Sequences
DNA
human CREBBP protein
In Vitro Techniques
Base Pairing
Assays
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

The coactivator CBP stimulates human T-cell lymphotrophic virus type I tax transactivation in vitro. / Kashanchi, Fatah; Duvall, Janet F.; Kwok, Roland P S; Lundblad, James; Goodman, Richard; Brady, John N.

In: Journal of Biological Chemistry, Vol. 273, No. 51, 18.12.1998, p. 34646-34652.

Research output: Contribution to journalArticle

Kashanchi, Fatah ; Duvall, Janet F. ; Kwok, Roland P S ; Lundblad, James ; Goodman, Richard ; Brady, John N. / The coactivator CBP stimulates human T-cell lymphotrophic virus type I tax transactivation in vitro. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 51. pp. 34646-34652.
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AU - Goodman, Richard

AU - Brady, John N.

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