The ClpX protein of Bacillus subtilis indirectly influences RNA polymerase holoenzyme composition and directly stimulates σ(H)-dependent transcription

Jiajian Liu, Peter Zuber

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40 Citations (Scopus)

Abstract

In Bacillus subtilis, several processes associated with the onset of stationary phase, including the initiation of sporulation, require the activity of the minor σ(H) form of RNA polymerase (RNAP). The induction of σ(H)-dependent gene transcription requires the regulatory ATPase, ClpX. The ClpX-dependent post-exponential increase in σ(H) activity is not dependent on the activator of sporulation gene expression, Spo0A. By determining the level of σ(H) and σ(A) in whole-cell extracts and RNAP preparations, evidence is presented that clpX does not influence the concentration of sigma subunits, but is required for the stationary phase reduction in σ(A)-RNAP holoenzyme. This is probably an indirect consequence of ClpX activity, because the ClpX-dependent decrease in σ(A)-RNAP concentration does not occur in a spo0A abrB mutant. The addition of ClpX to in vitro transcription reactions resulted in the stimulation of RNAP holoenzyme activity, but σ(H)-RNAP was observed to be more sensitive to ClpX-dependent stimulation than σ(A)-RNAP. No difference in transcriptional activity was observed in single-cycle in vitro transcription reactions, suggesting that ClpX acted at a step in transcription initiation after closed- and open-promoter complex formation. ClpX is proposed to function indirectly in the displacement of σ(A) from core RNAP and to act directly in the stimulation of σ(H)-dependent transcription in sporulating B. subtilis cells.

Original languageEnglish (US)
Pages (from-to)885-897
Number of pages13
JournalMolecular Microbiology
Volume37
Issue number4
DOIs
StatePublished - 2000

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Holoenzymes
DNA-Directed RNA Polymerases
Bacillus subtilis
Proteins
Sigma Factor
Cell Extracts
Adenosine Triphosphatases
Gene Expression

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

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title = "The ClpX protein of Bacillus subtilis indirectly influences RNA polymerase holoenzyme composition and directly stimulates σ(H)-dependent transcription",
abstract = "In Bacillus subtilis, several processes associated with the onset of stationary phase, including the initiation of sporulation, require the activity of the minor σ(H) form of RNA polymerase (RNAP). The induction of σ(H)-dependent gene transcription requires the regulatory ATPase, ClpX. The ClpX-dependent post-exponential increase in σ(H) activity is not dependent on the activator of sporulation gene expression, Spo0A. By determining the level of σ(H) and σ(A) in whole-cell extracts and RNAP preparations, evidence is presented that clpX does not influence the concentration of sigma subunits, but is required for the stationary phase reduction in σ(A)-RNAP holoenzyme. This is probably an indirect consequence of ClpX activity, because the ClpX-dependent decrease in σ(A)-RNAP concentration does not occur in a spo0A abrB mutant. The addition of ClpX to in vitro transcription reactions resulted in the stimulation of RNAP holoenzyme activity, but σ(H)-RNAP was observed to be more sensitive to ClpX-dependent stimulation than σ(A)-RNAP. No difference in transcriptional activity was observed in single-cycle in vitro transcription reactions, suggesting that ClpX acted at a step in transcription initiation after closed- and open-promoter complex formation. ClpX is proposed to function indirectly in the displacement of σ(A) from core RNAP and to act directly in the stimulation of σ(H)-dependent transcription in sporulating B. subtilis cells.",
author = "Jiajian Liu and Peter Zuber",
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journal = "Molecular Microbiology",
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T1 - The ClpX protein of Bacillus subtilis indirectly influences RNA polymerase holoenzyme composition and directly stimulates σ(H)-dependent transcription

AU - Liu, Jiajian

AU - Zuber, Peter

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N2 - In Bacillus subtilis, several processes associated with the onset of stationary phase, including the initiation of sporulation, require the activity of the minor σ(H) form of RNA polymerase (RNAP). The induction of σ(H)-dependent gene transcription requires the regulatory ATPase, ClpX. The ClpX-dependent post-exponential increase in σ(H) activity is not dependent on the activator of sporulation gene expression, Spo0A. By determining the level of σ(H) and σ(A) in whole-cell extracts and RNAP preparations, evidence is presented that clpX does not influence the concentration of sigma subunits, but is required for the stationary phase reduction in σ(A)-RNAP holoenzyme. This is probably an indirect consequence of ClpX activity, because the ClpX-dependent decrease in σ(A)-RNAP concentration does not occur in a spo0A abrB mutant. The addition of ClpX to in vitro transcription reactions resulted in the stimulation of RNAP holoenzyme activity, but σ(H)-RNAP was observed to be more sensitive to ClpX-dependent stimulation than σ(A)-RNAP. No difference in transcriptional activity was observed in single-cycle in vitro transcription reactions, suggesting that ClpX acted at a step in transcription initiation after closed- and open-promoter complex formation. ClpX is proposed to function indirectly in the displacement of σ(A) from core RNAP and to act directly in the stimulation of σ(H)-dependent transcription in sporulating B. subtilis cells.

AB - In Bacillus subtilis, several processes associated with the onset of stationary phase, including the initiation of sporulation, require the activity of the minor σ(H) form of RNA polymerase (RNAP). The induction of σ(H)-dependent gene transcription requires the regulatory ATPase, ClpX. The ClpX-dependent post-exponential increase in σ(H) activity is not dependent on the activator of sporulation gene expression, Spo0A. By determining the level of σ(H) and σ(A) in whole-cell extracts and RNAP preparations, evidence is presented that clpX does not influence the concentration of sigma subunits, but is required for the stationary phase reduction in σ(A)-RNAP holoenzyme. This is probably an indirect consequence of ClpX activity, because the ClpX-dependent decrease in σ(A)-RNAP concentration does not occur in a spo0A abrB mutant. The addition of ClpX to in vitro transcription reactions resulted in the stimulation of RNAP holoenzyme activity, but σ(H)-RNAP was observed to be more sensitive to ClpX-dependent stimulation than σ(A)-RNAP. No difference in transcriptional activity was observed in single-cycle in vitro transcription reactions, suggesting that ClpX acted at a step in transcription initiation after closed- and open-promoter complex formation. ClpX is proposed to function indirectly in the displacement of σ(A) from core RNAP and to act directly in the stimulation of σ(H)-dependent transcription in sporulating B. subtilis cells.

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