The ClpX chaperone modulates assembly of the tubulin-like protein FtsZ

Richard B. Weart, Shunji Nakano, Brooke E. Lane, Peter Zuber, Petra Anne Levin

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Assembly of the tubulin-like cytoskeletal protein FtsZ into a ring structure establishes the location of the nascent division site in prokaryotes. Factors that modulate FtsZ assembly are essential for ensuring the precise spatial and temporal regulation of cytokinesis. We have identified ClpX, the substrate recognition subunit of the ClpXP protease, as an inhibitor of FtsZ assembly in Bacillus subtilis. Genetic data indicate that ClpX but not ClpP inhibits FtsZ-ring formation in vivo. In vitro, ClpX inhibits FtsZ assembly in a ClpP-independent manner through a mechanism that does not require ATP hydrolysis. Together our data support a model in which ClpX helps maintain the cytoplasmic pool of unassembled FtsZ that is required for the dynamic nature of the cytokinetic ring. ClpX is conserved throughout bacteria and has been shown to interact directly with FtsZ in Escherichia coli. Thus, we speculate that ClpX functions as a general regulator of FtsZ assembly and cell division in a wide variety of bacteria.

Original languageEnglish (US)
Pages (from-to)238-249
Number of pages12
JournalMolecular Microbiology
Volume57
Issue number1
DOIs
StatePublished - Jul 2005

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Tubulin
Bacteria
Cytoskeletal Proteins
Cytokinesis
Bacillus subtilis
Protease Inhibitors
Cell Division
Proteins
Hydrolysis
Adenosine Triphosphate
Escherichia coli
In Vitro Techniques

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

The ClpX chaperone modulates assembly of the tubulin-like protein FtsZ. / Weart, Richard B.; Nakano, Shunji; Lane, Brooke E.; Zuber, Peter; Levin, Petra Anne.

In: Molecular Microbiology, Vol. 57, No. 1, 07.2005, p. 238-249.

Research output: Contribution to journalArticle

Weart, Richard B. ; Nakano, Shunji ; Lane, Brooke E. ; Zuber, Peter ; Levin, Petra Anne. / The ClpX chaperone modulates assembly of the tubulin-like protein FtsZ. In: Molecular Microbiology. 2005 ; Vol. 57, No. 1. pp. 238-249.
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