The cis-acting RNA trafficking signal from myelin basic protein mRNA and its cognate trans-acting ligand hnRNP A2 enhance cap-dependent translation

Sunjong Kwon, Elisa Barbarese, John H. Carson

Research output: Contribution to journalArticle

74 Scopus citations

Abstract

The 21 nucleotide RNA trafficking signal (RTS), originally identified in myelin basic protein mRNA, but also found in a variety of other localized RNAs, is necessary and sufficient for transport of RNA along microtubules in oligodendrocytes. The RTS binds specifically to the RNA binding protein, hnRNP A2. Together, the RTS and hnRNP A2 comprise cis/trans determinants for several steps in the RNA trafficking pathway. Here we show that insertion of the RTS into green fluorescent protein (GFP) RNA enhances translation without affecting stability of microinjected RNA. In dicistronic RNA, the RTS enhances cap-dependent translation without affecting internal ribosome entry site (IRES)-dependent translation. The translation enhancer function of the RTS is position, copy number, and cell type independent, hnRNP A2 dependent, and saturable with increasing amounts of injected RNA. This represents one of the first specific translation enhancer elements identified in a mammalian system.

Original languageEnglish (US)
Pages (from-to)247-256
Number of pages10
JournalJournal of Cell Biology
Volume147
Issue number2
DOIs
StatePublished - 1999

Keywords

  • Dicistronic RNA
  • HnRNP A2
  • RNA trafficking signal
  • Translation enhancer
  • eIF4E

ASJC Scopus subject areas

  • Cell Biology

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