The chondrogenic potential of human bone-marrow-derived mesenchymal progenitor cells

Jung Yoo, Traci S. Barthel, Keita Nishimura, Luis Solchaga, Arnold I. Caplan, Victor M. Goldberg, Brian Johnstone

Research output: Contribution to journalArticle

718 Citations (Scopus)

Abstract

Mesenchymal progenitor cells provide a source of cells for the repair of musculoskeletal tissue. However, in vitro models are needed to study the mechanisms of differentiation of progenitor cells. This study demonstrated the successful induction of in vitro chondrogenesis with human bone-marrow- derived osteochondral progenitor cells in a reliable and reproducible culture system. Human bone marrow was removed and fractionated, and adherent cell cultures were established. The cells were then passaged into an aggregate culture system in a serum-free medium. Initially, the cell aggregates contained type-I collagen and neither type-II nor type-X collagen was detected. Type-II collagen was typically detected in the matrix by the fifth day, with the immunoreactivity localized in the region of metachromatic staining. By the fourteenth day, type-II and type-X collagen were detected throughout the cell aggregates, except for an outer region of flattened, perichondrial-like cells in a matrix rich in type-I collagen. Aggrecan and link protein were detected in extracts of the cell aggregates, providing evidence that large aggregating proteoglycans of the type found in cartilaginous tissues had been synthesized by the newly differentiating chondrocytic cells; the small proteoglycans, biglycan and decorin, were also detected in extracts. Immunohistochemical staining with antibodies specific for chondroitin 4-sulfate and keratan sulfate demonstrated a uniform distribution of proteoglycans throughout the extracellular matrix of the cell aggregates. When the bone-marrow-derived cell preparations were passaged in monolayer culture as many as twenty times, with cells allowed to grow to confluence at each passage, the chondrogenic potential of the cells was maintained after each passage. CLINICAL RELEVANCE: Chondrogenesis of progenitor cells is the foundation for the in vivo repair of fractures and damaged articular cartilage. In vitro chondrogenesis of human bone-marrow- derived osteochondral progenitor cells should provide a useful model for studying this cellular differentiation. Furthermore, the maintenance of chondrogenic potential after greater than a billion-fold expansion provides evidence for the clinical utility of these cells in the repair of bone and cartilage.

Original languageEnglish (US)
Pages (from-to)1745-1757
Number of pages13
JournalJournal of Bone and Joint Surgery - Series A
Volume80
Issue number12
StatePublished - 1998

Fingerprint

Mesenchymal Stromal Cells
Bone Marrow
Chondrogenesis
Collagen Type II
Proteoglycans
Stem Cells
Collagen Type X
Biglycan
Staining and Labeling
Decorin
Keratan Sulfate
Aggrecans
Chondroitin Sulfates
Serum-Free Culture Media
Articular Cartilage
Collagen Type I
Cell Extracts
Bone Marrow Cells
Cartilage
Extracellular Matrix

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine

Cite this

Yoo, J., Barthel, T. S., Nishimura, K., Solchaga, L., Caplan, A. I., Goldberg, V. M., & Johnstone, B. (1998). The chondrogenic potential of human bone-marrow-derived mesenchymal progenitor cells. Journal of Bone and Joint Surgery - Series A, 80(12), 1745-1757.

The chondrogenic potential of human bone-marrow-derived mesenchymal progenitor cells. / Yoo, Jung; Barthel, Traci S.; Nishimura, Keita; Solchaga, Luis; Caplan, Arnold I.; Goldberg, Victor M.; Johnstone, Brian.

In: Journal of Bone and Joint Surgery - Series A, Vol. 80, No. 12, 1998, p. 1745-1757.

Research output: Contribution to journalArticle

Yoo, J, Barthel, TS, Nishimura, K, Solchaga, L, Caplan, AI, Goldberg, VM & Johnstone, B 1998, 'The chondrogenic potential of human bone-marrow-derived mesenchymal progenitor cells', Journal of Bone and Joint Surgery - Series A, vol. 80, no. 12, pp. 1745-1757.
Yoo J, Barthel TS, Nishimura K, Solchaga L, Caplan AI, Goldberg VM et al. The chondrogenic potential of human bone-marrow-derived mesenchymal progenitor cells. Journal of Bone and Joint Surgery - Series A. 1998;80(12):1745-1757.
Yoo, Jung ; Barthel, Traci S. ; Nishimura, Keita ; Solchaga, Luis ; Caplan, Arnold I. ; Goldberg, Victor M. ; Johnstone, Brian. / The chondrogenic potential of human bone-marrow-derived mesenchymal progenitor cells. In: Journal of Bone and Joint Surgery - Series A. 1998 ; Vol. 80, No. 12. pp. 1745-1757.
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