The CD59 family member leaky/coiled is required for the establishment of the blood-brain barrier in Drosophila

Mubarak Hussain Syed, Alice Krudewig, Daniel Engelen, Wilm Tobias Stork, Christian Klämbt

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The blood- brain barrier of Drosophila is established by the subperineurial glial cells that encase the CNS and PNS. The subperineurial glial cells are thin, highly interdigitated cells with epithelial character. The establishment of extensive septate junctions between these cells is crucial for the prevention of uncontrolled paracellular leakage of ions and solutes from the hemolymph into the nervous system. In the absence of septate junctions, macromolecules such as fluorescently labeled dextran can easily cross the blood- brain barrier. To identify additional components of the blood- brain barrier, we followed a genetic approach and injected Texas-Red-conjugated dextran into the hemolymph of embryos homozygous for chromosomal deficiencies. In this way, we identified the 153-aa-large protein Coiled, a new member of the Ly6 (leukocyte antigen 6) family, as being crucially required for septate junction formation and blood- brain barrier integrity. In coiled mutants, the normal distribution of septate junction markers such as Neurexin IV, Coracle, or Discs large is disturbed. EM analyses demonstrated that Coiled is required for the formation of septate junctions. We further show that Coiled is expressed by the subsperineurial glial cells in which it is anchored to the cell membrane via a glycosylphosphatidylinositol anchor and mediates adhesive properties. Clonal rescue studies indicate that the presence of Coiled is required symmetrically on both cells engaged in septate junction formation.

Original languageEnglish (US)
Pages (from-to)7876-7885
Number of pages10
JournalJournal of Neuroscience
Volume31
Issue number21
DOIs
StatePublished - May 25 2011
Externally publishedYes

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Blood-Brain Barrier
Drosophila
Neuroglia
Hemolymph
Dextrans
Glycosylphosphatidylinositols
Intercellular Junctions
Normal Distribution
HLA Antigens
Adhesives
Nervous System
Embryonic Structures
Epithelial Cells
Cell Membrane
Ions
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The CD59 family member leaky/coiled is required for the establishment of the blood-brain barrier in Drosophila. / Syed, Mubarak Hussain; Krudewig, Alice; Engelen, Daniel; Stork, Wilm Tobias; Klämbt, Christian.

In: Journal of Neuroscience, Vol. 31, No. 21, 25.05.2011, p. 7876-7885.

Research output: Contribution to journalArticle

Syed, Mubarak Hussain ; Krudewig, Alice ; Engelen, Daniel ; Stork, Wilm Tobias ; Klämbt, Christian. / The CD59 family member leaky/coiled is required for the establishment of the blood-brain barrier in Drosophila. In: Journal of Neuroscience. 2011 ; Vol. 31, No. 21. pp. 7876-7885.
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