The catechol 1,2 dioxygenase system of Acinetobacter radioresistens: Isoenzymes, inductors and gene localisation

E. Pessione, M. G. Giuffrida, R. Mazzoli, Patrizia Caposio, S. Landolfo, A. Conti, C. Giunta, G. Gribaudo

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Two different isozymes (Iso A and Iso B) of catechol 1,2 dioxygenase (C1,20) were isolated from cultures of A. radioresistens grown in two different media, containing phenol and benzoate respectively. In the phenol medium the bacteria expressed about 90% of Iso A, whereas in the benzoate medium the Iso A/Iso B ratio was 40:60. The two proteins have different molecular masses, isoelectric points and N-terminal sequences that are not consistent with simple post-translational modifications. Furthermore, their behaviour differs at high temperatures (42 °C-47 °C) and at moderately acidic pH (pH 6.0): Iso A proved to be the more stable under conditions of environmental stress. Hybridisation analysis with an A. calcoaceticus catA-derived probe revealed that A. radioresistens C1,20 proteins are encoded by two chromosomally located genes. Bidimensional electrophoresis (2DE) maps of crude extracts of cells grown in different carbon sources (phenol, benzoate and acetate) clearly demonstrated a differential induction pattern for the two proteins. The hypothesis of a double set of genes, one for benzoate catabolism and the other for phenol catabolism, is discussed, and analogies are drawn with other known C1,20s.

Original languageEnglish (US)
Pages (from-to)1253-1261
Number of pages9
JournalBiological Chemistry
Volume382
Issue number8
DOIs
StatePublished - 2001
Externally publishedYes

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Catechol 1,2-Dioxygenase
Acinetobacter
Benzoates
Phenol
Isoenzymes
Genes
Proteins
Isoelectric Point
Molecular mass
Post Translational Protein Processing
Electrophoresis
Complex Mixtures
Bacteria
Acetates
Carbon
Cells
Temperature

Keywords

  • 2DE
  • Benzoate
  • N-terminal sequence comparison
  • Phenol
  • Southern blot analysis

ASJC Scopus subject areas

  • Biochemistry

Cite this

The catechol 1,2 dioxygenase system of Acinetobacter radioresistens : Isoenzymes, inductors and gene localisation. / Pessione, E.; Giuffrida, M. G.; Mazzoli, R.; Caposio, Patrizia; Landolfo, S.; Conti, A.; Giunta, C.; Gribaudo, G.

In: Biological Chemistry, Vol. 382, No. 8, 2001, p. 1253-1261.

Research output: Contribution to journalArticle

Pessione, E, Giuffrida, MG, Mazzoli, R, Caposio, P, Landolfo, S, Conti, A, Giunta, C & Gribaudo, G 2001, 'The catechol 1,2 dioxygenase system of Acinetobacter radioresistens: Isoenzymes, inductors and gene localisation', Biological Chemistry, vol. 382, no. 8, pp. 1253-1261. https://doi.org/10.1515/BC.2001.156
Pessione, E. ; Giuffrida, M. G. ; Mazzoli, R. ; Caposio, Patrizia ; Landolfo, S. ; Conti, A. ; Giunta, C. ; Gribaudo, G. / The catechol 1,2 dioxygenase system of Acinetobacter radioresistens : Isoenzymes, inductors and gene localisation. In: Biological Chemistry. 2001 ; Vol. 382, No. 8. pp. 1253-1261.
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