Abstract
Rhabdomyosarcomas of the parotid and submandibular glands have the histological appearance of a skeletal muscle tumor yet can be found in tissue with no striated muscular elements. We examine the potential cell-of-origin for rhabdomyosarcoma and whether salivary tumors represent primary malignancy or metastasis. We have previously established genetically engineered mouse models of rhabdomyosarcoma. In these mice, rhabdomyosarcoma is only induced when a Pax3:Foxo1 fusion oncogene is activated with concurrent loss of p53 function (for alveolar rhabdomyosarcoma) or loss of p53 function alone (for embryonal rhabdomyosarcoma) using Cre-lox technology. These mutations are only activated under the control of promoters specific for selected cell lineages, previously thought to be myogenesis-restricted. RT-PCR and immunohistochemistry for lineage-specific promoter gene products reveal these promoters are active in wild-type mouse salivary gland. Given that mouse rhabdomyosarcoma frequently originates in the salivary glands and these myogenic-related promoters are normally expressed in salivary tissue, a high likelihood exists that the salivary gland contains a cell-of-origin of this muscle-related cancer.
| Original language | English (US) |
|---|---|
| Article number | 74 |
| Journal | Frontiers in Oncology |
| Volume | 5 |
| Issue number | APR |
| DOIs | |
| State | Published - 2015 |
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Keywords
- Head and neck oncology
- Oncogenesis
- Rhabdomyosarcoma
- Salivary gland
- Salivary gland pathology
- Sarcoma
- Tumor biology
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
The case for primary salivary rhabdomyosarcoma. / Geltzeiler, Mathew; Li, Guangheng; Abraham, Jinu; Keller, Charles.
In: Frontiers in Oncology, Vol. 5, No. APR, 74, 2015.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The case for primary salivary rhabdomyosarcoma
AU - Geltzeiler, Mathew
AU - Li, Guangheng
AU - Abraham, Jinu
AU - Keller, Charles
PY - 2015
Y1 - 2015
N2 - Rhabdomyosarcomas of the parotid and submandibular glands have the histological appearance of a skeletal muscle tumor yet can be found in tissue with no striated muscular elements. We examine the potential cell-of-origin for rhabdomyosarcoma and whether salivary tumors represent primary malignancy or metastasis. We have previously established genetically engineered mouse models of rhabdomyosarcoma. In these mice, rhabdomyosarcoma is only induced when a Pax3:Foxo1 fusion oncogene is activated with concurrent loss of p53 function (for alveolar rhabdomyosarcoma) or loss of p53 function alone (for embryonal rhabdomyosarcoma) using Cre-lox technology. These mutations are only activated under the control of promoters specific for selected cell lineages, previously thought to be myogenesis-restricted. RT-PCR and immunohistochemistry for lineage-specific promoter gene products reveal these promoters are active in wild-type mouse salivary gland. Given that mouse rhabdomyosarcoma frequently originates in the salivary glands and these myogenic-related promoters are normally expressed in salivary tissue, a high likelihood exists that the salivary gland contains a cell-of-origin of this muscle-related cancer.
AB - Rhabdomyosarcomas of the parotid and submandibular glands have the histological appearance of a skeletal muscle tumor yet can be found in tissue with no striated muscular elements. We examine the potential cell-of-origin for rhabdomyosarcoma and whether salivary tumors represent primary malignancy or metastasis. We have previously established genetically engineered mouse models of rhabdomyosarcoma. In these mice, rhabdomyosarcoma is only induced when a Pax3:Foxo1 fusion oncogene is activated with concurrent loss of p53 function (for alveolar rhabdomyosarcoma) or loss of p53 function alone (for embryonal rhabdomyosarcoma) using Cre-lox technology. These mutations are only activated under the control of promoters specific for selected cell lineages, previously thought to be myogenesis-restricted. RT-PCR and immunohistochemistry for lineage-specific promoter gene products reveal these promoters are active in wild-type mouse salivary gland. Given that mouse rhabdomyosarcoma frequently originates in the salivary glands and these myogenic-related promoters are normally expressed in salivary tissue, a high likelihood exists that the salivary gland contains a cell-of-origin of this muscle-related cancer.
KW - Head and neck oncology
KW - Oncogenesis
KW - Rhabdomyosarcoma
KW - Salivary gland
KW - Salivary gland pathology
KW - Sarcoma
KW - Tumor biology
UR - http://www.scopus.com/inward/record.url?scp=84934282441&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84934282441&partnerID=8YFLogxK
U2 - 10.3389/fonc.2015.00074
DO - 10.3389/fonc.2015.00074
M3 - Article
VL - 5
JO - Frontiers in Oncology
JF - Frontiers in Oncology
SN - 2234-943X
IS - APR
M1 - 74
ER -