The capacity of UL49.5 proteins to inhibit TAP is widely distributed among members of the genus varicellovirus

Marieke Verweij, Andrea D. Lipińska, Danijela Koppers-Lalic, Wouter F. Van Leeuwen, Jeffrey I. Cohen, Paul R. Kinchington, Ilhem Messaoudi, Krystyna Bieńkowska-Szewczyk, Maaike E. Ressing, Frans A M Rijsewijk, Emmanuel J H J Wiertz

Research output: Contribution to journalArticle

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Abstract

The lifelong infection by varicelloviruses is characterized by a fine balance between the host immune response and immune evasion strategies used by these viruses. Virus-derived peptides are presented to cytotoxic T lymphocytes by major histocompatibility complex (MHC) class I molecules. The transporter associated with antigen processing (TAP) transports the peptides from the cytosol into the endoplasmic reticulum, where the loading of MHC-I molecules occurs. The varicelloviruses bovine herpesvirus 1 (BoHV-1), pseudorabies virus, and equid herpesviruses 1 and 4 have been found to encode a UL49.5 protein that inhibits TAP-mediated peptide transport. To investigate to what extent UL49.5-mediated TAP inhibition is conserved within the family of Alphaherpesvirinae, the homologs of another five varicelloviruses, one mardivirus, and one iltovirus were studied. The UL49.5 proteins of BoHV-5, bubaline herpesvirus 1, cervid herpesvirus 1, and felid herpesvirus 1 were identified as potent TAP inhibitors. The varicella-zoster virus and simian varicellovirus UL49.5 proteins fail to block TAP; this is not due to the absence of viral cofactors that might assist in this process, since cells infected with these viruses did not show reduced TAP function either. The UL49.5 homologs of the mardivirus Marek's disease virus 1 and the iltovirus infectious laryngotracheitis virus did not block TAP, suggesting that the capacity to inhibit TAP via UL49.5 has been acquired by varicelloviruses only. A phylogenetic analysis of viruses that inhibit TAP through their UL49.5 proteins reveals an interesting hereditary pattern, pointing toward the presence of this capacity in defined clades within the genus Varicellovirus.

Original languageEnglish (US)
Pages (from-to)2351-2363
Number of pages13
JournalJournal of Virology
Volume85
Issue number5
DOIs
StatePublished - Mar 2011

Fingerprint

Varicellovirus
Iltovirus
trypsinogen activation peptide
Viruses
Mardivirus
Herpesviridae
viruses
Proteins
proteins
major histocompatibility complex
peptides
Major Histocompatibility Complex
Bubaline herpesvirus 1
Cervid herpesvirus 1
Alphaherpesvirinae
Equid Herpesvirus 4
Bovine herpesvirus 5
Equid Herpesvirus 1
Equid herpesvirus 4
Human herpesvirus 3

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Verweij, M., Lipińska, A. D., Koppers-Lalic, D., Van Leeuwen, W. F., Cohen, J. I., Kinchington, P. R., ... Wiertz, E. J. H. J. (2011). The capacity of UL49.5 proteins to inhibit TAP is widely distributed among members of the genus varicellovirus. Journal of Virology, 85(5), 2351-2363. https://doi.org/10.1128/JVI.01621-10

The capacity of UL49.5 proteins to inhibit TAP is widely distributed among members of the genus varicellovirus. / Verweij, Marieke; Lipińska, Andrea D.; Koppers-Lalic, Danijela; Van Leeuwen, Wouter F.; Cohen, Jeffrey I.; Kinchington, Paul R.; Messaoudi, Ilhem; Bieńkowska-Szewczyk, Krystyna; Ressing, Maaike E.; Rijsewijk, Frans A M; Wiertz, Emmanuel J H J.

In: Journal of Virology, Vol. 85, No. 5, 03.2011, p. 2351-2363.

Research output: Contribution to journalArticle

Verweij, M, Lipińska, AD, Koppers-Lalic, D, Van Leeuwen, WF, Cohen, JI, Kinchington, PR, Messaoudi, I, Bieńkowska-Szewczyk, K, Ressing, ME, Rijsewijk, FAM & Wiertz, EJHJ 2011, 'The capacity of UL49.5 proteins to inhibit TAP is widely distributed among members of the genus varicellovirus', Journal of Virology, vol. 85, no. 5, pp. 2351-2363. https://doi.org/10.1128/JVI.01621-10
Verweij M, Lipińska AD, Koppers-Lalic D, Van Leeuwen WF, Cohen JI, Kinchington PR et al. The capacity of UL49.5 proteins to inhibit TAP is widely distributed among members of the genus varicellovirus. Journal of Virology. 2011 Mar;85(5):2351-2363. https://doi.org/10.1128/JVI.01621-10
Verweij, Marieke ; Lipińska, Andrea D. ; Koppers-Lalic, Danijela ; Van Leeuwen, Wouter F. ; Cohen, Jeffrey I. ; Kinchington, Paul R. ; Messaoudi, Ilhem ; Bieńkowska-Szewczyk, Krystyna ; Ressing, Maaike E. ; Rijsewijk, Frans A M ; Wiertz, Emmanuel J H J. / The capacity of UL49.5 proteins to inhibit TAP is widely distributed among members of the genus varicellovirus. In: Journal of Virology. 2011 ; Vol. 85, No. 5. pp. 2351-2363.
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