The Caenorhabditis elegans ABL-1 tyrosine kinase is required for Shigella flexneri pathogenesis

Elizabeth A. Burton, Ann Marie Pendergast, Alejandro Aballay

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Shigellosis is a diarrheal disease caused by the gram-negative bacterium Shigella flexneri. Following ingestion of the bacterium, S. flexneri interferes with innate immunity, establishes an infection within the human colon, and initiates an inflammatory response that results in destruction of the tissue lining the gut. Examination of host cell factors required for S. flexneri pathogenesis in vivo has proven difficult due to limited host susceptibility. Here we report the development of a pathogenesis system that involves the use of Caenorhabditis elegans as a model organism to study S. flexneri virulence determinants and host molecules required for pathogenesis. We show that S. flexneri-mediated killing of C. elegans correlates with bacterial accumulation in the intestinal tract of the animal. The S. flexneri virulence plasmid, which encodes a type III secretory system as well as various virulence determinants crucial for pathogenesis in mammalian systems, was found to be required for maximal C. elegans killing. Additionally, we demonstrate that ABL-1, the C. elegans homolog of the mammalian c-Abl nonreceptor tyrosine kinase ABL1, is required for S. flexneri pathogenesis in nematodes. These data demonstrate the feasibility of using C. elegans to study S. flexneri pathogenesis in vivo and provide insight into host factors that contribute to S. flexneri pathogenesis.

Original languageEnglish (US)
Pages (from-to)5043-5051
Number of pages9
JournalApplied and Environmental Microbiology
Volume72
Issue number7
DOIs
StatePublished - Jul 1 2006
Externally publishedYes

Fingerprint

Shigella flexneri
Caenorhabditis elegans
virulence
Protein-Tyrosine Kinases
tyrosine
phosphotransferases (kinases)
pathogenesis
diarrheal disease
bacterium
immunity
plasmid
nematode
Virulence
animal
shigellosis
Bacillary Dysentery
Gram-Negative Bacteria
Innate Immunity
Gram-negative bacteria
colon

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

The Caenorhabditis elegans ABL-1 tyrosine kinase is required for Shigella flexneri pathogenesis. / Burton, Elizabeth A.; Pendergast, Ann Marie; Aballay, Alejandro.

In: Applied and Environmental Microbiology, Vol. 72, No. 7, 01.07.2006, p. 5043-5051.

Research output: Contribution to journalArticle

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