The autoreactive T cell population in experimental allergic encephalomyelitis: T cell receptor β-chain rearrangements

Mary Pat Happ, Aniko S. Kiraly, Halina Offner, Arthur Vandenbark, Ellen Heber-Katz

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

T cell receptor β-chain gene rearrangements were examined in myelin basic protein (MBP)-reactive T cell lines and hybridomas from Lewis (Lew) rats. Nearly 75% of the cloned hybrids were specific for the major encephalitogenic determinant residues 68-88 of guinea pig (GP) MBP; three fine specificities could be distinguished. Southern blot analysis of receptor β-chain genes revealed polyclonality and shared rearrangements not seen in non-68-88-specific clones. Generation of short-term, encephalitogenic Lew T cell lines revealed rearrangements shared with the 68-88-specific hybrids, indicating that the hybrids were representative of the whole antigen-specific population and suggesting restricted Vβ gene usage within this polyclonal population.

Original languageEnglish (US)
Pages (from-to)191-204
Number of pages14
JournalJournal of Neuroimmunology
Volume19
Issue number3
DOIs
StatePublished - Sep 1988

Keywords

  • (Guinea pig)
  • Experimental allergic encephalomyelitis
  • Major histocompatibility complex
  • Myelin basic protein
  • T cell receptor β-chain

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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