Abstract
T cell receptor β-chain gene rearrangements were examined in myelin basic protein (MBP)-reactive T cell lines and hybridomas from Lewis (Lew) rats. Nearly 75% of the cloned hybrids were specific for the major encephalitogenic determinant residues 68-88 of guinea pig (GP) MBP; three fine specificities could be distinguished. Southern blot analysis of receptor β-chain genes revealed polyclonality and shared rearrangements not seen in non-68-88-specific clones. Generation of short-term, encephalitogenic Lew T cell lines revealed rearrangements shared with the 68-88-specific hybrids, indicating that the hybrids were representative of the whole antigen-specific population and suggesting restricted Vβ gene usage within this polyclonal population.
Original language | English (US) |
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Pages (from-to) | 191-204 |
Number of pages | 14 |
Journal | Journal of Neuroimmunology |
Volume | 19 |
Issue number | 3 |
DOIs | |
State | Published - Sep 1988 |
Externally published | Yes |
Keywords
- (Guinea pig)
- Experimental allergic encephalomyelitis
- Major histocompatibility complex
- Myelin basic protein
- T cell receptor β-chain
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Neurology
- Clinical Neurology