The autoreactive T cell population in experimental allergic encephalomyelitis: T cell receptor β-chain rearrangements

Mary Pat Happ, Aniko S. Kiraly, Halina Offner, Arthur Vandenbark, Ellen Heber-Katz

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

T cell receptor β-chain gene rearrangements were examined in myelin basic protein (MBP)-reactive T cell lines and hybridomas from Lewis (Lew) rats. Nearly 75% of the cloned hybrids were specific for the major encephalitogenic determinant residues 68-88 of guinea pig (GP) MBP; three fine specificities could be distinguished. Southern blot analysis of receptor β-chain genes revealed polyclonality and shared rearrangements not seen in non-68-88-specific clones. Generation of short-term, encephalitogenic Lew T cell lines revealed rearrangements shared with the 68-88-specific hybrids, indicating that the hybrids were representative of the whole antigen-specific population and suggesting restricted Vβ gene usage within this polyclonal population.

Original languageEnglish (US)
Pages (from-to)191-204
Number of pages14
JournalJournal of Neuroimmunology
Volume19
Issue number3
DOIs
StatePublished - 1988
Externally publishedYes

Fingerprint

Autoimmune Experimental Encephalomyelitis
T-Cell Antigen Receptor
Myelin Basic Protein
T-Lymphocytes
Population
T-Cell Receptor Genes
Cell Line
Gene Rearrangement
Hybridomas
Southern Blotting
Genes
Guinea Pigs
Clone Cells
Antigens

Keywords

  • (Guinea pig)
  • Experimental allergic encephalomyelitis
  • Major histocompatibility complex
  • Myelin basic protein
  • T cell receptor β-chain

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Clinical Neurology
  • Neurology

Cite this

The autoreactive T cell population in experimental allergic encephalomyelitis : T cell receptor β-chain rearrangements. / Happ, Mary Pat; Kiraly, Aniko S.; Offner, Halina; Vandenbark, Arthur; Heber-Katz, Ellen.

In: Journal of Neuroimmunology, Vol. 19, No. 3, 1988, p. 191-204.

Research output: Contribution to journalArticle

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