The antineoplastic effect of vitamin D in transgenic mice with retinoblastoma

D. M. Albert, D. M. Marcus, J. P. Gallo, J. M. O'Brien

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Vitamin D has been shown to inhibit growth of human retinoblastoma in tissue culture and nude mouse heterografts. We have described a heritable transgenic mouse model of retinoblastoma. The in vivo efficacy of 1,25- dihydroxycholecalciferol (vitamin D3) was examined by administering this agent to transgenic mice with retinoblastoma. Forty-six 8-10-week-old transgene-bearing mice were injected intraperitoneally for 5 wk. Experimental animals received 0.05 μg (15 animals) or 0.025 μg (15 animals) of vitamin D. Sixteen control animals received only a mineral oil vehicle. Eyes were enucleated at 5 mo and were examined histologically by two investigators in a masked fashion. All control animals demonstrated bilateral involvement of retinoblastoma. Four eyes in the low-dose group and six eyes in the high-dose group had no evidence of retinoblastoma. Eyes treated with vitamin D3 showed less extensive involvement of the retina by retinoblastoma. Vitamin D-treated animals demonstrated tumors confined to the retina, whereas control animals demonstrated larger tumors, more often invading the vitreous, anterior chamber, and choroid. Thus, Vitamin D inhibited the growth and local extension in a dose-dependent fashion.

Original languageEnglish (US)
Pages (from-to)2354-2364
Number of pages11
JournalInvestigative Ophthalmology and Visual Science
Volume33
Issue number8
StatePublished - 1992

Keywords

  • 1,25 dihydroxycholecalciferol
  • angiogenesis
  • retinoblastoma
  • transgenic mice
  • vitamin D

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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